ALKBH4 Antibody (YA2050)(PBS only)

Cat. No.: HY-P82305A: Data Sheet User Guide for Antibodies
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ALKBH4 Antibody (YA2050) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALKBH4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ALKBH4 Antibody (YA2050) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALKBH4.

Host

Rabbit

Molecular Weight

Predicted band size: 34 kDa;

Observed band size: 34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NXW9

Gene ID

54784 [NCBI]

Immunogen

Recombinant protein of human ALKBH4 aa2-302.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed:23673617). Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed:23673617). In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity)

Subcellular Localization：Cytoplasm; Nucleus; Nucleus, nucleolus; Midbody

Expression：
Tissue_specificity:Widely expressed, with highest expression in pancreas, ovary and spleen

Isoforms & Post-Translational Modification：Q9NXW9 has 2 isomers: Q9NXW9-1: 33838 Da (predicted); Q9NXW9-2: 4556 Da (predicted).

Subunit：Interacts with ZFHX3, MLLT3, MLLT1, HSF4, EP300, TES, EIF3C, MTMR6 and PSMA6

Research Field

Epigenetics and Nuclear Signaling

Synonyms

ABH4

Documentation

User Guide for Antibodies (1077 KB)