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  5. Survivin/BIRC5 Antibody (YA5423)

Survivin/BIRC5 Antibody (YA5423)

製品番号: HY-P85731
User Guide for Antibodies Technical Support

Survivin/BIRC5 Antibody (YA5423) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Survivin/BIRC5.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Survivin/BIRC5 Antibody (YA5423) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Survivin/BIRC5.
Host

Mouse
Clonality

Monoclonal
分子量
Observed band size: 16 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Rat
SwissProt ID
Gene ID
Immunogen

Recombinant Protein of Survivin
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-200
純度 affinity chromatography. Conjugation Non-conjugated
Modification Unmodified  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:20627126, PubMed:21364656, PubMed:25778398, PubMed:28218735, PubMed:9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797)
Subcellular Localization：Cytoplasm; Nucleus; Chromosome; Chromosome, centromere; Cytoplasm, cytoskeleton, spindle; Chromosome, centromere, kinetochore; Midbody
Expression：
Tissue_specificity:It is expressed only in the fetal kidney and liver, and less frequently in the lung and brain (PubMed: 10626797) . It is highly expressed in adenocarcinomas (lung, pancreatic, colon, breast, and prostate cancers) and high-grade lymphomas (PubMed: 14741722, PubMed: 16329164) . It is also expressed in various renal cell carcinoma lines (PubMed: 10626797) . It is expressed in the cochlea, including the organ of Corti, lateral walls, marginal interdental cells, as well as Schwann cells, cochlear neurons, and spiral ganglion cells (protein level) . It is not expressed in the internal sulcus, external sulcus cells, or Reissner's membrane cells (protein level) (PubMed: 20627126, PubMed: 21364656) .

Induction:Up-regulated by COMP
Isoforms & Post-Translational Modification：O15392 has 7 isomers: O15392-1: 16389 Da (predicted); O15392-2: 18636 Da (predicted); O15392-3: 15622 Da (predicted); O15392-4: 13812 Da (predicted); O15392-5: 13467 Da (predicted); O15392-6: 8991 Da (predicted); O15392-7: 8490 Da (predicted).
Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting. Deubiquitinated by USP35 or USP38; leading to stabilization (PubMed:34438346);In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074). Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625). Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247). Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis;Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation
Subunit：Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer.
Synonyms
BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin
ドキュメンテーション
User Guide for Antibodies (1077 KB)

Survivin/BIRC5 Antibody (YA5423) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
Survivin/BIRC5 Antibody (YA5423)
製品番号:
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