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PGC1 alpha+beta Antibody (YA3458)

(Synonyms: LEM6 antibody; Ligand effect modulator 6 antibody; PERC antibody; Peroxisome proliferative activated receptor, gamma, coactivator 1 antibody; PGC 1 alpha antibody; PGC-1-beta antibody; PGC-1-related estrogen receptor alpha coactivator antibody; PGC1 antibody; PGC1A antibody; PGC1v antibody; PPAR gamma coactivator 1 alpha antibody; PPAR gamma coactivator-1 beta antibody; PPARGC 1 alpha antibody; PPARGC-1-beta antibody; PPARGC1 antibody; PPARGC1A antibody; Ppargc1b antibody; PRGC1_HUMAN antibody; PRGC2_HUMAN antibody; )
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Based on 1 Customer Validation

PGC1 alpha+beta Antibody (YA3458) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGC1 alpha+beta.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PGC1 alpha+beta Antibody (YA3458)
  • PGC1 alpha+beta Antibody (YA3458) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:2000

Product Details

Description

PGC1 alpha+beta Antibody (YA3458) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGC1 alpha+beta.

  • Host Rabbit
  • Clonality Monoclonal, Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 113 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 113 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within Human PGC1 alpha aa 755-798 /798 and aa 269-323 /798.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676641

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PGC1 alpha+beta Antibody (YA3458)
    Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using PGC1 alpha+beta Antibody (HY-P83728) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PGC1 alpha+beta is a Q86YN6: Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner
    Q9UBK2: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.
    [1][2][3][4][5].

  • Subcellular Localization

    Q86YN6: Nucleus
    Q9UBK2: Nucleus; Nucleus, PML body; Nucleus; Cytoplasm; Nucleus; Nucleus; Nucleus, PML body; Nucleus

  • Expression


    Tissue_specificity: Q86YN6: Ubiquitous with higher expression in heart, brain and skeletal muscle
    Q9UBK2: Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects
    Induction: Q86YN6: Repressed by saturated fatty acids such as palmitate and stearate in skeletal muscle cells. Induced by insulin and reduced by aging in skeletal muscle biopsies. Down-regulated in type 2 diabetes mellitus subjects as well as in pre-diabetics
    Q9UBK2: Transcription is repressed by ZNF746 which binds to 'insulin response sequences' its promoter

  • Isoforms & Post-Translational Modification

    Q86YN6 has 6 isomers: Q86YN6-1: 113222 Da (predicted); Q86YN6-2: 110981 Da (predicted); Q86YN6-3: 112568 Da (predicted); Q86YN6-4: 110327 Da (predicted); Q86YN6-5: 108919 Da (predicted); Q86YN6-6: 106230 Da (predicted).

  • Subunit

    Q86YN6: Interacts with hepatocyte nuclear factor 4-alpha/HNF4A, Sterol regulatory binding transcription factor 1/SREBF1, PPAR-alpha/PPARA, thyroid hormone receptor beta/THRB and host cell factor/HCFC1. Interacts with estrogen-related receptor gamma/ESRRG and alpha/ESRRA. Interacts with PRDM16 (By similarity). Interacts with estrogen receptor alpha/ESR1
    Q9UBK2: Homooligomer (PubMed:10713165).

  • SwissProt ID

    Q86YN6 ，Q9UBK2

  • Gene ID
    10891 [NCBI]
  • Synonyms

    LEM6 antibody; Ligand effect modulator 6 antibody; PERC antibody; Peroxisome proliferative activated receptor, gamma, coactivator 1 antibody; PGC 1 alpha antibody; PGC-1-beta antibody; PGC-1-related estrogen receptor alpha coactivator antibody; PGC1 antibody; PGC1A antibody; PGC1v antibody; PPAR gamma coactivator 1 alpha antibody; PPAR gamma coactivator-1 beta antibody; PPARGC 1 alpha antibody; PPARGC-1-beta antibody; PPARGC1 antibody; PPARGC1A antibody; Ppargc1b antibody; PRGC1_HUMAN antibody; PRGC2_HUMAN antibody;

PGC1 alpha+beta Antibody (YA3458) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using PGC1 alpha+beta Antibody (HY-P83728) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PGC1 alpha+beta Antibody (YA3458) Product Image
    1/16
  • Experimental Validation Results for PGC1 alpha+beta Antibody (YA3458)
  • PGC1 alpha+beta Antibody (YA3458) Product Image
  • Experimental Validation Results for PGC1 alpha+beta Antibody (YA3458)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using PGC1 alpha+beta Antibody (HY-P83728) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PGC1 alpha+beta Antibody (YA3458)
  • PGC1 alpha+beta Antibody (YA3458) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using PGC1 alpha+beta Antibody (HY-P83728) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PGC1 alpha+beta Antibody (YA3458) Product Image WB