Nucleostemin Antibody (YA4462)(PBS only)

Art. -Nr.: HY-P84765A: Data Sheet User Guide for Antibodies
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Nucleostemin Antibody (YA4462) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Nucleostemin.

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100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

Nucleostemin Antibody (YA4462) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Nucleostemin.

Host

Mouse

Molekulargewicht

Predicted band size: 62 kDa;

Observed band size: 62 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9BVP2

Gene ID

26354 [NCBI]

Immunogen

Purified recombinant fragment of human GNL3 (AA: 1-226) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Reinheit	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity)

Subcellular Localization：Nucleus; Nucleus, nucleolus

Expression：
Tissue_specificity:The level of this substance is elevated in cancerous lung tissue.

Isoforms & Post-Translational Modification：Q9BVP2 has 2 isomers: Q9BVP2-1: 61993 Da (predicted); Q9BVP2-2: 60540 Da (predicted).

Subunit：Interacts with MDM2; this interaction stabilizes MDM2 (By similarity). Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly (By similarity). Indirectly interacts with TP53, via MDM2-binding (PubMed:12464630). Interacts with TSC22D1 isoform 2 (PubMed:34681573)

Synonyms

NS; E2IG3; NNP47; C77032

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/lu-99a-cell.html