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Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only)

(Synonyms: mitochondrial; ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex; E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 com)

Pyruvate Dehydrogenase E1 alpha Antibody (YA681) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 alpha.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only)
  • Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Pyruvate Dehydrogenase E1 alpha Antibody (YA681) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 alpha.

  • Host Mouse
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Immunogen

Synthetic peptide corresponding to Human Pyruvate Dehydrogenase E1 alpha.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only)
    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), and 293T (lane 4, 15 μg) using Pyruvate Dehydrogenase E1 alpha Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Pyruvate Dehydrogenase E1 alpha is a The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Tissue_specificity:general expression

  • Isoforms & Post-Translational Modification

    P08559 has 4 isomers: P08559-1: 43296 Da (predicted); P08559-2: 44109 Da (predicted); P08559-3: 40188 Da (predicted); P08559-4: 47580 Da (predicted).
    Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation;Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity)

  • Subunit

    Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules

  • SwissProt ID

    P08559

  • Gene ID
    5160 [NCBI]
  • Synonyms

    mitochondrial; ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex; E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 com

  • Research Field

    Signal Transduction

Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), and 293T (lane 4, 15 μg) using Pyruvate Dehydrogenase E1 alpha Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only)
  • Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only) Product Image
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), and 293T (lane 4, 15 μg) using Pyruvate Dehydrogenase E1 alpha Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only)
  • Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), and 293T (lane 4, 15 μg) using Pyruvate Dehydrogenase E1 alpha Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Pyruvate Dehydrogenase E1 alpha Antibody (YA681)(PBS only) Product Image WB