HTR3B Antibody (YA3570)(PBS only)

Cat. No.: HY-P83873A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

HTR3B Antibody (YA3570) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to HTR3B.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HTR3B Antibody (YA3570) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to HTR3B.

Host

Mouse

Molecular Weight

Predicted band size: 50 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95264

Gene ID

9177 [NCBI]

Immunogen

Purified recombinant fragment of human HTR3B (AA: extra 23-150) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons

Subcellular Localization：Postsynaptic cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Expressed in the brain cortex, in the caudate nucleus, the hippocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta

Isoforms & Post-Translational Modification：O95264 has 2 isomers: O95264-1: 50292 Da (predicted); O95264-2: 49285 Da (predicted).
N-glycosylation required for membrane localization

Subunit：Forms homopentameric as well as heteropentameric serotonin-activated cation-selective channel complexes with HTR3A. The homomeric complex is not functional. Heteropentameric complexes display properties which resemble that of neuronal serotonin-activated channels in vivo

Synonyms

5-HT3B

Documentation

User Guide for Antibodies (1077 KB)