Phospho-c-Jun (S73)+JunD (S100) Antibody (YA9720)
(Synonyms: Activator protein 1, AP 1, AP-1, AP1, cJun, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, JUN, Jun oncogene, JUN protein)Phospho-c-Jun (S73)+JunD (S100) Antibody (YA9720) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to Phospho-c-Jun(S73)+JunD(S100) .
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, ICC/IF, FC
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:100 | 1:1000 |
Product Details
Phospho-c-Jun (S73)+JunD (S100) Antibody (YA9720) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to Phospho-c-Jun(S73)+JunD(S100) .
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman, Mouse, Rat
-
Calculated Molecular Weight Predicted band size: 40/45 kDa;
Synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
c-Jun +JunD is a Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells[1][2][3][4][5].
-
Subcellular Localization
Nucleus
-
Expression
Tissue_Specificity: Expressed in the developing and adult prostate and prostate cancer cells. -
Isoforms & Post-Translational Modification
Phospho-c-Jun+JunD has an amino acid length of 331, molecular weight is 35676 Da.
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. -
Subunit
Heterodimer with either BATF3 or ATF7.
-
SwissProt ID
-
Synonyms
Activator protein 1, AP 1, AP-1, AP1, cJun, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, JUN, Jun oncogene, JUN protein
Documentation
References
[1]. Qing J, et al. Structural and functional characterization of the transforming growth factor-beta -induced Smad3/c-Jun transcriptional cooperativity. J Biol Chem. 2000 Dec 8;275(49):38802-12. [Content Brief]
[2]. Ji Z, et al. The forkhead transcription factor FOXK2 promotes AP-1-mediated transcriptional regulation. Mol Cell Biol. 2012 Jan;32(2):385-98. [Content Brief]
[3]. Baumann S, et al. An unexpected role for FosB in activation-induced cell death of T cells. Oncogene. 2003 Mar 6;22(9):1333-9. [Content Brief]
[4]. Lan HC, et al. Cyclic AMP stimulates SF-1-dependent CYP11A1 expression through homeodomain-interacting protein kinase 3-mediated Jun N-terminal kinase and c-Jun phosphorylation. Mol Cell Biol. 2007 Mar;27(6):2027-36. [Content Brief]
[5]. Serra RW, et al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife. 2014 Mar 12;3:e02313. [Content Brief]
Powered by Bioz
