PARD3 Antibody
(Synonyms: PAR3, PAR3A, PARD3, Partitioning defective 3 homolog, PAR-3, PARD-3, Atypical PKC isotype-specific-interacting protein, CTCL tumor antigen se2-5, PAR3-alpha, ASIP)PARD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PARD3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
PARD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PARD3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 180 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 151 kDa
Synthetic peptide corresponding to the C-term region of human PARD3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PARD3 is an Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Involved in Schwann cell peripheral myelination (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (By similarity). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons[1][2][3][4].
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Subcellular Localization
Cytoplasm; Endomembrane system; Cell junction; Cell junction, tight junction; Cell junction, adherens junction; Cell membrane; Cytoplasm, cell cortex; Cytoplasm, cytoskeleton
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Expression
Tissue_Specificity: Widely expressed. -
Isoforms & Post-Translational Modification
PARD3 has 11 isoforms, Q8TEW0-1: amino acid length is 1356, molecular weight is 151423 Da (predicted); Q8TEW0-2: amino acid length is 1353, molecular weight is 151150 Da (predicted); Q8TEW0-3: amino acid length is 1266, molecular weight is 141072 Da (predicted); Q8TEW0-4: amino acid length is 1319, molecular weight is 146877 Da (predicted); Q8TEW0-5: amino acid length is 1244, molecular weight is 138332 Da (predicted); Q8TEW0-6: amino acid length is 1310, molecular weight is 146276 Da (predicted); Q8TEW0-7: amino acid length is 1273, molecular weight is 141730 Da (predicted); Q8TEW0-8: amino acid length is 1031, molecular weight is 113419 Da (predicted); Q8TEW0-9: amino acid length is 988, molecular weight is 108545 Da (predicted); Q8TEW0-10: amino acid length is 1000, molecular weight is 109917 Da (predicted); Q8TEW0-11: amino acid length is 1340, molecular weight is 149695 Da (predicted).发生乙酰化修饰。可被 SIRT2 去乙酰化，从而抑制施万细胞的外周髓鞘形成PARD3 存在 11 个异构体，Q8TEW0-1：氨基酸个数为 1356 个，分子量为 151423 Da (预测)；Q8TEW0-2：氨基酸个数为 1353 个，分子量为 151150 Da (预测)；Q8TEW0-3：氨基酸个数为 1266 个，分子量为 141072 Da (预测)；Q8TEW0-4：氨基酸个数为 1319 个，分子量为 146877 Da (预测)；Q8TEW0-5：氨基酸个数为 1244 个，分子量为 138332 Da (预测)；Q8TEW0-6：氨基酸个数为 1310 个，分子量为 146276 Da (预测)；Q8TEW0-7：氨基酸个数为 1273 个，分子量为 141730 Da (预测)；Q8TEW0-8：氨基酸个数为 1031 个，分子量为 113419 Da (预测)；Q8TEW0-9：氨基酸个数为 988 个，分子量为 108545 Da (预测)；Q8TEW0-10：氨基酸个数为 1000 个，分子量为 109917 Da (预测)；Q8TEW0-11：氨基酸个数为 1340 个，分子量为 149695 Da (预测)。Acetylated. Deacetylated by SIRT2, thereby inhibiting Schwann cell peripheral myelination
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Subunit
Interacts (via PDZ 1 domain) with F11R/JAM1, PARD6A and PARD6B.
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SwissProt ID
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Synonyms
PAR3, PAR3A, PARD3, Partitioning defective 3 homolog, PAR-3, PARD-3, Atypical PKC isotype-specific-interacting protein, CTCL tumor antigen se2-5, PAR3-alpha, ASIP
Documentation
[1]. Johansson A, et al. The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1. J Cell Sci. 2000 Sep;113 ( Pt 18):3267-75. [Content Brief]
[2]. Chen X, et al. Rare Deleterious PARD3 Variants in the aPKC-Binding Region are Implicated in the Pathogenesis of Human Cranial Neural Tube Defects Via Disrupting Apical Tight Junction Formation. Hum Mutat. 2017 Apr;38(4):378-389. [Content Brief]
[3]. Joberty G, et al. The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42. Nat Cell Biol. 2000 Aug;2(8):531-9. [Content Brief]
[4]. Khazaei MR, et al. Phosphorylation of the par polarity complex protein Par3 at serine 962 is mediated by aurora a and regulates its function in neuronal polarity. J Biol Chem. 2009 Nov 27;284(48):33571-9. [Content Brief]
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