MTO1 Antibody (YA8981)
(Synonyms: CGI 02, CGI-02, Protein MTO1 homolog, mitochondrial)MTO1 Antibody (YA8981) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MTO1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:100-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:30-3000 |
Product Details
MTO1 Antibody (YA8981) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MTO1.
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Host Mouse
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Species Reactivityhuman
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Observed Molecular WeightObserved band size: 90 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 80 kDa
A synthesized peptide derived from human MTO1.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
MTO1 is a Component of the GTPBP3-MTO1 complex that catalyzes the 5-taurinomethyluridine (taum(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs (mt-tRNAs), which plays a role in mt-tRNA decoding and mitochondrial translation. Taum(5)U formation on mammalian mt-tRNA requires the presence of both GTPBP3-mediated GTPase activity and MTO1 catalytic activity[1][2].
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Subcellular Localization
Mitochondrion
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Expression
Tissue_Specificity: Ubiquitously expressed in various tissues, but with a markedly elevated expression in tissues of high metabolic rates including cochlea. -
Isoforms & Post-Translational Modification
MTO1 has 6 isoforms, Q9Y2Z2-1: amino acid length is 717, molecular weight is 79964 Da (predicted); Q9Y2Z2-2: amino acid length is 595, molecular weight is 66842 Da (predicted); Q9Y2Z2-3: amino acid length is 313, molecular weight is 34295 Da (predicted); Q9Y2Z2-4: amino acid length is 692, molecular weight is 77301 Da (predicted); Q9Y2Z2-5: amino acid length is 643, molecular weight is 72047 Da (predicted); Q9Y2Z2-6: amino acid length is 732, molecular weight is 81538 Da (predicted).
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Subunit
Homodimer; forms a dimer in the presence of potassium.
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SwissProt ID
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Synonyms
CGI 02, CGI-02, Protein MTO1 homolog, mitochondrial
Documentation
References
[1]. Asano K, et al. Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. Nucleic Acids Res. 2018 Feb 28;46(4):1565-1583. [Content Brief]
[2]. Peng GX, et al. The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. Nucleic Acids Res. 2021 Mar 18;49(5):2816-2834. [Content Brief]
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