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L1CAM Antibody (YA5135)

(Synonyms: L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171)

L1CAM Antibody (YA5135) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to L1CAM.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for L1CAM Antibody (YA5135)
  • L1CAM Antibody (YA5135) Product Image
  • Experimental Validation Results for L1CAM Antibody (YA5135)
  • L1CAM Antibody (YA5135) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

제품 설명

L1CAM Antibody (YA5135) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to L1CAM.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 250 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 140 kDa
Immunogen

Full length human recombinant protein of human L1CAM produced in 293T cell.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • 선적

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for L1CAM Antibody (YA5135)
    Flow cytometric analysis of 1X106 HeLa cells labeling L1CAM Antibody(HY-P85443, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, growth cone; Cell projection, axon; Cell projection, dendrite

  • Isoforms & Post-Translational Modification

    P32004 has 3 isomers: P32004-1: 140003 Da (predicted); P32004-2: 139517 Da (predicted); P32004-3: 138908 Da (predicted).

  • Subunit

    Interacts with SHTN1; the interaction occurs in axonal growth cones (By similarity). Interacts with isoform 2 of BSG (By similarity)

  • SwissProt ID

    P32004

  • Gene ID
    3897 [NCBI]
  • Synonyms

    L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171

L1CAM Antibody (YA5135) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    L1CAM Antibody (YA5135) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling L1CAM Antibody(HY-P85443, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • L1CAM Antibody (YA5135) Product Image
    1/16
  • Experimental Validation Results for L1CAM Antibody (YA5135)
  • L1CAM Antibody (YA5135) Product Image
  • L1CAM Antibody (YA5135) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling L1CAM Antibody(HY-P85443, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • L1CAM Antibody (YA5135) Product Image FC