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SV2A Antibody (YA6783)

(Synonyms: KIAA0736 antibody; OTTHUMP00000014065 antibody; SV2 antibody; Sv2a antibody; SV2A_HUMAN antibody; Synaptic vesicle glycoprotein 2 antibody; Synaptic vesicle glycoprotein 2A antibody; Synaptic vesicle protein 2a antibody; )
Customer Review

Based on 1 Customer Validation

SV2A Antibody (YA6783) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SV2A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SV2A Antibody (YA6783)
  • Experimental Validation Results for SV2A Antibody (YA6783)
  • SV2A Antibody (YA6783) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000 1:500-1:2000 1:500 1-2μg/sample

Product Details

Description

SV2A Antibody (YA6783) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SV2A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Cynomolgus monkey,Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 83 kDa
Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719258

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SV2A Antibody (YA6783)
    Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) and Rat brain (lane 3(20μg)) using SV2A Antibody (HY-P87090) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SV2A Antibody (YA6783)
    Western blot analysis was performed on protein extracts (30 μg) from Mouse brain (lane 2) and Rat brain (lane 3) using SV2A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    SV2A plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|(Microbial infection) Receptor for the C.botulinum neurotoxin type A2 (BoNT/A, botA); glycosylation is not essential but enhances the interaction. Probably also serves as a receptor for the closely related C.botulinum neurotoxin type A1[1].

  • Subcellular Localization

    Presynapse,Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane

  • Isoforms & Post-Translational Modification

    Q7L0J3 has two isomers: Q7L0J3-1: 82695 Da (predicted); Q7L0J3-2: 76619 Da (predicted).
    Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1丨N-glycosylated

  • Subunit

    Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner

  • SwissProt ID

    Q7L0J3

  • Gene ID
    9900 [NCBI]
  • Synonyms

    KIAA0736 antibody; OTTHUMP00000014065 antibody; SV2 antibody; Sv2a antibody; SV2A_HUMAN antibody; Synaptic vesicle glycoprotein 2 antibody; Synaptic vesicle glycoprotein 2A antibody; Synaptic vesicle protein 2a antibody;

SV2A Antibody (YA6783) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) and Rat brain (lane 3(20μg)) using SV2A Antibody (HY-P87090) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Mouse brain (lane 2) and Rat brain (lane 3) using SV2A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • SV2A Antibody (YA6783) Product Image
    1/16
  • Experimental Validation Results for SV2A Antibody (YA6783)
  • Experimental Validation Results for SV2A Antibody (YA6783)
  • SV2A Antibody (YA6783) Product Image
  • Experimental Validation Results for SV2A Antibody (YA6783)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) and Rat brain (lane 3(20μg)) using SV2A Antibody (HY-P87090) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SV2A Antibody (YA6783)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Mouse brain (lane 2) and Rat brain (lane 3) using SV2A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for SV2A Antibody (YA6783)
  • Experimental Validation Results for SV2A Antibody (YA6783)
  • SV2A Antibody (YA6783) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) and Rat brain (lane 3(20μg)) using SV2A Antibody (HY-P87090) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SV2A Antibody (YA6783) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Mouse brain (lane 2) and Rat brain (lane 3) using SV2A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • SV2A Antibody (YA6783) Product Image WB
  • SV2A Antibody (YA6783) Product Image WB