CEP55 Antibody (YA2797)(PBS only)

Cat. No.: HY-P83052A: Data Sheet User Guide for Antibodies
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CEP55 Antibody (YA2797) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEP55.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CEP55 Antibody (YA2797) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEP55.

Host

Rabbit

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q53EZ4

Gene ID

55165 [NCBI]

Immunogen

A synthetic peptide of human CEP55 aa 17-30.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893). Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893). Not required for microtubule nucleation (PubMed:16198290). Plays a role in the development of the brain and kidney (PubMed:28264986)

Subcellular Localization：Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cleavage furrow; Midbody, Midbody ring

Expression：
Tissue_specificity:Expressed in embryonic brain (PubMed:28264986) . Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex (PubMed:28264986) . Expressed in human brain, cerebellum, kidney tubules, intestine and muscles (at protein level) (PubMed:28264986, PubMed:28295209) . Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in human thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain

Isoforms & Post-Translational Modification：Q53EZ4 has 2 isomers: Q53EZ4-1: 54178 Da (predicted); Q53EZ4-2: 46857 Da (predicted).
There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully

Subunit：Homodimer (PubMed:16406728). Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1; the interaction is indirect via the MTMR3:MTMR4 heterooligomer, occurs during early mitosis, regulates the phosphorylation of CEP55 by PLK1 and its recruitment to the midbody where it can mediate cell abscission (PubMed:16198290, PubMed:25659891). Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site (PubMed:17556548, PubMed:17853893, PubMed:18641129, PubMed:18948538). Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding (PubMed:17556548, PubMed:17853893, PubMed:18948538). Interacts with MVB12A, VPS37B, VPS37C and VPS28 (PubMed:17853893)

Research Field

Cell Biology

Synonyms

CEP55; C10orf3; URCC6; Centrosomal protein of 55 kDa; Cep55; Up-regulated in colon cancer 6

Documentation

User Guide for Antibodies (1077 KB)