CD1A Antibody (YA3664)
(Synonyms: R4; T6; CD1; FCB6; HTA1)
Based on 1 Customer Validation
CD1A Antibody (YA3664) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD1A.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
WB, IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse, Monkey
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
CD1A Antibody (YA3664) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD1A.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Monkey
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Observed Molecular WeightObserved band size: 37 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 37 kDa
Purified recombinant fragment of human CD1A (AA: 17-116) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from MOLT4(lane 1(40μg)and Raji(lane 2(40μg) ) using CD1a antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature. -
Flow cytometric analysis of 1X10^6 MOLT4 cells labeling CD1a Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray). -
Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD1a Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray). -
Immunofluorescence analysis of Hela cells labeling CD1a antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD1a antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue) -
Immunofluorescence analysis of NIH3T3 cells labeling CD1a antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD1a antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
Background
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Function
CD1a is an MHC-class-I-like antigen-presenting molecule that binds lipid antigens and activates CD1-restricted T cells[1]. Mechanistically, CD1 proteins bind hydrophobic lipid chains in antigen-binding grooves and position polar headgroups for TCR recognition[2]. In human skin, Langerhans cells express very high levels of CD1a, making CD1a lipid antigen presentation a central pathway for studying skin-resident T-cell activation[3]. CD1a-reactive T cells form a normal human skin T-cell population, and CD1a tetramers without added antigen stained approximately 1% of skin T cells in every tested subject[4]. In disease models, human CD1a transgenic mice showed stronger systemic inflammation after imiquimod- or MC903-induced skin inflammation, and CD1a-blocking antibodies reduced CD1a-dependent inflammatory responses[5]. Compared with related isoforms, CD1a belongs to group 1 CD1 molecules with CD1b and CD1c, whereas CD1d belongs to group 2 and presents lipids to iNKT cells[6]. CD1b and CD1c survey distinct intracellular compartments, supporting isoform-specific lipid antigen sampling rather than redundant CD1 function[7]. For experimental applications, CD1a tetramers enable tracking of CD1a-specific T cells, while inhibitory natural skin lipids and CD1a-blocking antibodies provide tools to test CD1a-dependent inflammation[4][5][8].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein; Endosome membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:It is expressed in cortical thymocytes, epidermal Langerhans cells, dendritic cells, certain T-cell leukemia cells, and various other tissues. -
Subunit
Heterodimer with B2M (beta-2-microglobulin). Interacts with CD74
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SwissProt ID
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Synonyms
R4; T6; CD1; FCB6; HTA1
Documentation
[1]. Barral DC, et al. CD1 antigen presentation: how it works. Nat Rev Immunol. 2007 Dec;7(12):929-41. [Content Brief]
[2]. Porcelli SA, et al. The CD1 system: antigen-presenting molecules for T cell recognition of lipids and glycolipids. Annu Rev Immunol. 1999;17:297-329. [Content Brief]
[3]. Ogg GS, et al. CD1a and bound lipids drive T-cell responses in human skin disease. Eur J Immunol. 2023 Oct;53(10):e2250333. [Content Brief]
[4]. Cotton RN, et al. Human skin is colonized by T cells that recognize CD1a independently of lipid. J Clin Invest. 2021;131(1):e140706. [Content Brief]
[5]. Hardman CS, et al. CD1a promotes systemic manifestations of skin inflammation. Nat Commun. 2022 Dec 7;13(1):7535. [Content Brief]
[6]. Genardi S, et al. CD1-Restricted T Cells in Inflammatory Skin Diseases. J Invest Dermatol. 2022 Mar;142(3 Pt B):768-773. [Content Brief]
[7]. Briken V, et al. Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens. J Exp Med. 2000 Jul 17;192(2):281-8. [Content Brief]
[8]. Fahmy LM, et al. CD1a and Lipids as Mediators of T Cell Activation in the Skin. J Invest Dermatol. 2025 Oct;145(10):2428-2435. [Content Brief]
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