ELK3 Antibody (YA8467)

Cat. No.: HY-P88783: Data Sheet COA
SDS
User Guide for Antibodies
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ELK3 Antibody (YA8467) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ELK3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

ELK3 Antibody (YA8467) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ELK3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 44.1 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P41970

Immunogen

Full length human recombinant protein of human ELK3 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of HeLa cells labeling ELK3 Antibody (HY-P88783) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELK3 Antibody (HY-P88783) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：P41970: 407 amino acids, molecular weight 44240 Da.

Subunit：Interacts with CTBP1

Synonyms

ERP; NET; SAP-2; SAP2

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/pc-9-cell.html [Content Brief]