MARK2 Antibody (YA2104)
(Synonyms: ELKL motif kinase 1 antibody; ELKL motif kinase antibody; EMK-1 antibody; EMK1 antibody; MAP/microtubule affinity regulating kinase 2 antibody; MAP/microtubule affinity-regulating kinase 2 antibody; Mark2 antibody; MARK2_HUMAN antibody; MGC99619 antibody; PAR 1 antibody; Par 1b antibody; PAR1 homolog antibody; Par1b antibody; Ser/Thr protein kinase PAR 1B antibody; Serine/threonine protein kinase EMK antibody; Serine/threonine protein kinase MARK2 antibody; Serine/threonine-protein kinase MARK2 antibody; )MARK2 Antibody (YA2104) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 |
Product Details
MARK2 Antibody (YA2104) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK2.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 78, 82 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 88 kDa
Entrez Gene: 2011 Human ; 13728 Mouse ; 60328 Rat
SwissProt: Q7KZI7 Human ; Q05512 Mouse ; O08679 Rat
OMIM: 600526 Human
A synthesized peptide derived from human MARK2 aa700-788.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Versand
Shipping with blue ice.
Background
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Function
MARK2 (Microtubule Affinity-Regulating Kinase 2) is a serine/threonine kinase that modulates microtubule dynamics by phosphorylating microtubule-associated proteins (MAPs)， thereby regulating cytoskeletal organization and cellular polarity[1][2]. Mechanistically, MARK2 phosphorylates specific sites within MAPs, including tau, which reduces microtubule binding and facilitates microtubule remodeling[3][1]. MARK2 activity influences multiple signaling pathways such as Hippo, mTOR, and NF-κB, connecting cytoskeletal regulation to cell proliferation, apoptosis, and intracellular transport[2]. In disease models, MARK2 dysregulation contributes to neurodegenerative conditions through tau hyperphosphorylation and is implicated in cancer progression via modulation of cell cycle and survival pathways[2]. Compared with related isoforms MARK1 and MARK4, MARK2 exhibits distinct substrate specificity and tissue expression patterns, enabling selective investigation of its cellular roles[1][2]. For experimental applications, selective inhibitors of MARK2 have been identified, including low-micromolar compounds based on 9-oxo-9H-acridin-10-yl scaffolds, which allow precise modulation of kinase activity in neuronal and cancer cell models[1]. These inhibitors are instrumental in studying microtubule dynamics, axon growth, and signal transduction with isoform-specific resolution[1]. MARK2 thus serves as a critical target for understanding cytoskeletal regulation in health and disease and for the development of kinase-targeted interventions[2].
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Subcellular Localization
Cell membrane; Peripheral membrane protein; Cytoplasm; Lateral cell membrane; Cytoplasm, cytoskeleton; Cell projection, dendrite; Cytoplasm
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Expression
Tissue_specificity:High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney -
Isoforms & Post-Translational Modification
Q7KZI7 has 16 isomers: Q7KZI7-1: 87911 Da (predicted); Q7KZI7-2: 78851 Da (predicted); Q7KZI7-3: 83184 Da (predicted); Q7KZI7-4: 81280 Da (predicted); Q7KZI7-5: 80647 Da (predicted); Q7KZI7-6: 84333 Da (predicted); Q7KZI7-7: 77702 Da (predicted); Q7KZI7-8: 86762 Da (predicted); Q7KZI7-9: 82429 Da (predicted); Q7KZI7-10: 77069 Da (predicted); Q7KZI7-11: 86129 Da (predicted); Q7KZI7-12: 82551 Da (predicted); Q7KZI7-13: 77631 Da (predicted); Q7KZI7-14: 83113 Da (predicted); Q7KZI7-15: 79427 Da (predicted); Q7KZI7-16: 81209 Da (predicted).
Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm -
Subunit
Homodimer. Interacts with PAK5; leading to inhibit the protein kinase activity (By similarity). Interacts with MAPT/TAU (PubMed:23666762). Interacts with MTCL1 isoform 1; the interaction is direct and increases MARK2 microtubule-binding ability (PubMed:23902687). Interacts (when phosphorylated at Thr-596) with YWHAZ (PubMed:15324659). Interacts with YWHAB, YWHAG and YWHAQ (PubMed:16959763)
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SwissProt ID
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Synonyms
ELKL motif kinase 1 antibody; ELKL motif kinase antibody; EMK-1 antibody; EMK1 antibody; MAP/microtubule affinity regulating kinase 2 antibody; MAP/microtubule affinity-regulating kinase 2 antibody; Mark2 antibody; MARK2_HUMAN antibody; MGC99619 antibody; PAR 1 antibody; Par 1b antibody; PAR1 homolog antibody; Par1b antibody; Ser/Thr protein kinase PAR 1B antibody; Serine/threonine protein kinase EMK antibody; Serine/threonine protein kinase MARK2 antibody; Serine/threonine-protein kinase MARK2 antibody;
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Research Field
Signal Transduction
Documentation
[1]. Jenkins SM, et al. Microtubule/MAP-affinity regulating kinase (MARK) is activated by phenylarsine oxide in situ and phosphorylates tau within its microtubule-binding domain. J Neurochem. 2000 Apr;74(4):1463-8. [Content Brief]
[2]. Timm T, et al. Microtubule affinity regulating kinase activity in living neurons was examined by a genetically encoded fluorescence resonance energy transfer/fluorescence lifetime imaging-based biosensor: inhibitors with therapeutic potential. J Biol Chem. 2011 Dec 2;286(48):41711-41722. [Content Brief]
[3]. Alam M, et al. Therapeutic targeting of microtubule affinity-regulating kinase 4 in cancer and neurodegenerative diseases. J Cell Biochem. 2023 Sep;124(9):1223-1240. [Content Brief]
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