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  5. MARK2 Antibody (YA2104)

MARK2 Antibody (YA2104)

Cat. No.: HY-P82359
User Guide for Antibodies Technical Support

MARK2 Antibody (YA2104) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK2.

For research use only. We do not sell to patients.

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20 μL Get quote 4 - 5 Weeks 3 - 4 Weeks 5 - 6 Weeks 4 - 5 weeks
50 μL Get quote 4 - 5 Weeks 3 - 4 Weeks 5 - 6 Weeks 4 - 5 weeks
100 μL Get quote 4 - 5 Weeks 3 - 4 Weeks 5 - 6 Weeks 4 - 5 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

MARK2 Antibody (YA2104) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK2.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 88 kDa;
Observed band size: 78, 82 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human MARK2 aa700-788.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells
Subcellular Localization：Cell membrane; Peripheral membrane protein; Cytoplasm; Lateral cell membrane; Cytoplasm, cytoskeleton; Cell projection, dendrite; Cytoplasm
Expression：
Tissue_specificity:High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney
Isoforms & Post-Translational Modification：Q7KZI7 has 16 isomers: Q7KZI7-1: 87911 Da (predicted); Q7KZI7-2: 78851 Da (predicted); Q7KZI7-3: 83184 Da (predicted); Q7KZI7-4: 81280 Da (predicted); Q7KZI7-5: 80647 Da (predicted); Q7KZI7-6: 84333 Da (predicted); Q7KZI7-7: 77702 Da (predicted); Q7KZI7-8: 86762 Da (predicted); Q7KZI7-9: 82429 Da (predicted); Q7KZI7-10: 77069 Da (predicted); Q7KZI7-11: 86129 Da (predicted); Q7KZI7-12: 82551 Da (predicted); Q7KZI7-13: 77631 Da (predicted); Q7KZI7-14: 83113 Da (predicted); Q7KZI7-15: 79427 Da (predicted); Q7KZI7-16: 81209 Da (predicted).
Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm
Subunit：Homodimer. Interacts with PAK5; leading to inhibit the protein kinase activity (By similarity). Interacts with MAPT/TAU (PubMed:23666762). Interacts with MTCL1 isoform 1; the interaction is direct and increases MARK2 microtubule-binding ability (PubMed:23902687). Interacts (when phosphorylated at Thr-596) with YWHAZ (PubMed:15324659). Interacts with YWHAB, YWHAG and YWHAQ (PubMed:16959763)
Database

Entrez Gene: 2011 Human ; 13728 Mouse ; 60328 Rat

SwissProt: Q7KZI7 Human ; Q05512 Mouse ; O08679 Rat

OMIM: 600526 Human

Research Field

Signal Transduction
Synonyms
ELKL motif kinase 1 antibody; ELKL motif kinase antibody; EMK-1 antibody; EMK1 antibody; MAP/microtubule affinity regulating kinase 2 antibody; MAP/microtubule affinity-regulating kinase 2 antibody; Mark2 antibody; MARK2_HUMAN antibody; MGC99619 antibody; PAR 1 antibody; Par 1b antibody; PAR1 homolog antibody; Par1b antibody; Ser/Thr protein kinase PAR 1B antibody; Serine/threonine protein kinase EMK antibody; Serine/threonine protein kinase MARK2 antibody; Serine/threonine-protein kinase MARK2 antibody;
Documentation
User Guide for Antibodies (1077 KB)

MARK2 Antibody (YA2104) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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