DDB1 Antibody (YA785)
(Synonyms: XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1)DDB1 Antibody (YA785) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to DDB1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat, Monkey
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Formulation:
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Antibody Dilution Buffer (for Western Blot)
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Ultra High Sensitivity ECL Kit
HY-K1005
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Hematoxylin-Eosin Staining Kit
HY-K0315
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3-Color Prestained Protein Marker (10-190 kDa)
HY-K1011
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High Sensitivity ECL Kit
HY-K2005
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Penicillin-Streptomycin (100×), Sterile
HY-K1006
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GelHP Protein Gel (Tris-Gly, 12%, 10 well)
HY-K1113
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DPBS Buffer (1×)
HY-K3008
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GelHP Protein Gel (Tris-Gly, 10%, 15 well)
HY-K1112
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Ponceau Staining Solution (Ready-to-use, 1×)
HY-K1109
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GelHP Protein Gel (Tris-Gly, 10%, 10 well)
HY-K1111
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
DDB1 Antibody (YA785) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to DDB1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat, Monkey
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Observed Molecular WeightObserved band size: 127 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 127 kDa
Entrez Gene: 1642 Human ; 13194 Mouse ; 64470 Rat
SwissProt: Q16531 Human ; Q3U1J4 Mouse ; Q9ESW0 Rat
OMIM: 619426 Human
Synthetic peptide corresponding to Human DDB1.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
DNA Damage Binding Protein-1 (DDB1) is a protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. The ubiquitin–proteasome system is a significant pathway that precisely governs protein stability. Among the Cullin-RING Ligase (CRL) family, a typical CRL4 ligase is composed of a scaffold Cullin (CUL4A or CUL4B) protein, a linker protein-DDB1, a substrate recognition adaptor (DCAF)， and a ring protein with intrinsic E3 activity (RBX1 or RBX2) .
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Subcellular Localization
Cytoplasm; Nucleus
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Isoforms & Post-Translational Modification
Q16531 has 2 isomers: Q16531-1: 126968 Da (predicted); Q16531-2: 50611 Da (predicted).
Phosphorylated by ABL1;Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis;Acetylated, promoting interaction with CUL4 (CUL4A or CUL4B) and subsequent formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes (PubMed:28886238). Deacetylation by SIRT7 impairs the interaction with CUL4 (CUL4A or CUL4B) and formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes (PubMed:28886238) -
Subunit
Component of the UV-DDB complex which includes DDB1 and DDB2; the heterodimer dimerizes to give rise to a heterotetramer when bound to damaged DNA (PubMed:16223728, PubMed:16527807, PubMed:19109893, PubMed:22822215, PubMed:9632823).
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SwissProt ID
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Synonyms
XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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