RNF14 Antibody (YA3216)
(Synonyms: RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein)RNF14 Antibody (YA3216) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RNF14.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
RNF14 Antibody (YA3216) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RNF14.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 54 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 54 kDa
Entrez Gene: 81847 Human ; 68031 Mouse ; 308051 Rat
SwissProt: Q9NTX7 Human ; Q9CZW6 Mouse ; Q5XIK5 Rat
OMIM: 612137 Human
A synthetic peptide of human RNF14
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RNF14 is an E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates 'Lys-6'-linked ubiquitination of target proteins, leading to their degradation. Mediates ubiquitination of EEF1A1/eEF1A and ETF1/eRF1 translation factors on stalled ribosomes, leading to their degradation. Also catalyzes ubiquitination of ribosomal proteins RPL0, RPL1, RPL12, RPS13 and RPS17. Specifically required to resolve RNA-protein cross-links caused by reactive aldehydes, which trigger translation stress by stalling ribosomes: acts by catalying 'Lys-6'-linked ubiquitination of RNA-protein cross-links, leading to their removal by the ATP-dependent unfoldase VCP and subsequent degradation by the proteasome. Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4). May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:Widely expressed -
Isoforms & Post-Translational Modification
Q9UBS8 has 2 isomers: Q9UBS8-1: 53837 Da (predicted); Q9UBS8-2: 39634 Da (predicted).
RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination -
Subunit
Interacts with GCN1; interaction takes place in response to ribosome collisions and is required for ubiquitination of EEF1A1/eEF1A (PubMed:36638793).
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SwissProt ID
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Synonyms
RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein
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Research Field
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Documentation
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