Copy Link Print Information

ATG7 Antibody (YA6201)

(Synonyms: Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein; )
Customer Review

Based on 1 Customer Validation

ATG7 Antibody (YA6201) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • ATG7 Antibody (YA6201) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

ATG7 Antibody (YA6201) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG7.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 78 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATG7 Antibody (YA6201)
    Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and C6(lane 4(20ug),3T3-L1(lane 5(20ug) using ATG7 Antibody (HY-P86509) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATG7 Antibody (YA6201)
    Immunocytochemistry analysis of HeLa cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATG7 Antibody (YA6201)
    Immunocytochemistry analysis of HT-29 cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Atg7 is a core autophagy-related protein with E1-like enzyme activity, responsible for activating and transferring Atg8 (LC3/GABARAP) family proteins to facilitate autophagosome formation. In addition to its role in classical autophagy, Atg7 is involved in immune regulation (such as LC3-associated phagocytosis, LAP), cell cycle control, apoptosis, and non-conventional protein secretion. It plays a crucial role in maintaining organelle homeostasis, removing damaged cellular components, and responding to cellular stress.
    Loss of Atg7 can lead to neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Moreover, Atg7 can both suppress tumor development and enhance tumor cell survival under extreme conditions. Atg7 also plays a significant role in infection defense and metabolic homeostasis, with its deficiency potentially increasing the risk of infections and being associated with metabolic diseases such as type 2 diabetes and fatty liver disease. Therefore, Atg7 is a potential research target for multiple diseases[1].

  • Subcellular Localization

    Cytoplasm; Preautophagosomal structure

  • Expression


    Tissue_specificity:It is widely expressed, especially in the kidneys, liver, lymph nodes, and bone marrow.

    Induction:Expression is up-regulated by the transcription factor HSF1

  • Isoforms & Post-Translational Modification

    O95352 has 3 isomers: O95352-1: 77960 Da (predicted); O95352-2: 75002 Da (predicted); O95352-3: 68618 Da (predicted).
    Acetylated by EP300;Polyubiquitinated on Lys-45 via 'Lys-63'-linked ubiquitin by TRIM32; this modification positiely regulates ATG8 and ATG12 activating enzyme activity leading to initiation of autophagy under metabolic stress

  • Subunit

    Homodimer (PubMed:11096062). Interacts with ATG3; this interaction is essential for the transfer of ATG8-like proteins's thioester from ATG7 to ATG3 and plays a role in the conjugation of ATG12 to ATG5 (PubMed:11825910, PubMed:26043688). Interacts with ATG12 (PubMed:11096062, PubMed:11825910). Forms intermediate conjugates with GABARAPL1 (By similarity). Forms intermediate conjugates with ATG8-like proteins such as GABARAP, GABARAPL2 or MAP1LC3A (PubMed:11096062, PubMed:16303767). Interacts with EP300 acetyltransferase (PubMed:19124466). Interacts with FOXO1 (PubMed:20543840)

  • SwissProt ID

    O95352

  • Gene ID
    10533 [NCBI]
  • Synonyms

    Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein;

References

[1]. Collier JJ, et al. Emerging roles of ATG7 in human health and disease. EMBO Mol Med. 2021 Dec 7;13(12):e14824. [Content Brief]

ATG7 Antibody (YA6201) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and C6(lane 4(20ug),3T3-L1(lane 5(20ug) using ATG7 Antibody (HY-P86509) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HT-29 cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATG7 Antibody (YA6201) Product Image
    1/16
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • ATG7 Antibody (YA6201) Product Image
  • Experimental Validation Results for ATG7 Antibody (YA6201)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and C6(lane 4(20ug),3T3-L1(lane 5(20ug) using ATG7 Antibody (HY-P86509) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATG7 Antibody (YA6201)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATG7 Antibody (YA6201)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HT-29 cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • Experimental Validation Results for ATG7 Antibody (YA6201)
  • ATG7 Antibody (YA6201) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and C6(lane 4(20ug),3T3-L1(lane 5(20ug) using ATG7 Antibody (HY-P86509) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ATG7 Antibody (YA6201) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATG7 Antibody (YA6201) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HT-29 cells labeling ATG7 with ATG7 Antibody (HY-P86509) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATG7 Antibody (HY-P86509) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATG7 Antibody (YA6201) Product Image WB
  • ATG7 Antibody (YA6201) Product Image ICC
  • ATG7 Antibody (YA6201) Product Image ICC