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eIF4A2 Antibody (YA6850)

(Synonyms: ATP dependent RNA helicase eIF4A 2 antibody; ATP-dependent RNA helicase eIF4A-2 antibody; BM-010 antibody; DDX2B antibody; eIF 4A II antibody; eIF-4A-II antibody; EIF4A antibody; eIF4A II antibody; eIF4A-II antibody; EIF4A2 antibody; ATP dependent RNA helicase eIF4A 2 antibody; ATP-dependent RNA helicase eIF4A-2 antibody; BM-010 antibody; DDX2B antibody; eIF 4A II antibody; eIF-4A-II antibody; EIF4A antibody; eIF4A II antibody; eIF4A-II antibody; EIF4A2 antibody; EIF4F antibody; Eukaryotic initiation factor 4A II antibody; Eukaryotic initiation factor 4A-II antibody; eukaryotic translation initiation factor 4A isoform 2 antibody; Eukaryotic translation initiation factor 4A2 antibody; IF4A2_HUMAN antibody; N-terminally processed antibody; )
Customer Review

Based on 1 Customer Validation

eIF4A2 Antibody (YA6850) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to eIF4A2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for eIF4A2 Antibody (YA6850)
  • eIF4A2 Antibody (YA6850) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:1000 1:100 1:1000

Product Details

Description

eIF4A2 Antibody (YA6850) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to eIF4A2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 46 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Immunogen

Recombinant protein within Human eIF4A2 aa 1-407 / 407.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719273

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for eIF4A2 Antibody (YA6850)
    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using eIF4A2 Antibody (HY-P87157) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    eIF4A2 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon

  • Isoforms & Post-Translational Modification

    Q14240 has two isomers: Q14240-1: 46402 Da (predicted); Q14240-2: 46489 Da (predicted).

  • Subunit

    eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions

  • SwissProt ID

    Q14240

  • Gene ID
    1974 [NCBI]
  • Synonyms

    ATP dependent RNA helicase eIF4A 2 antibody; ATP-dependent RNA helicase eIF4A-2 antibody; BM-010 antibody; DDX2B antibody; eIF 4A II antibody; eIF-4A-II antibody; EIF4A antibody; eIF4A II antibody; eIF4A-II antibody; EIF4A2 antibody; ATP dependent RNA helicase eIF4A 2 antibody; ATP-dependent RNA helicase eIF4A-2 antibody; BM-010 antibody; DDX2B antibody; eIF 4A II antibody; eIF-4A-II antibody; EIF4A antibody; eIF4A II antibody; eIF4A-II antibody; EIF4A2 antibody; EIF4F antibody; Eukaryotic initiation factor 4A II antibody; Eukaryotic initiation factor 4A-II antibody; eukaryotic translation initiation factor 4A isoform 2 antibody; Eukaryotic translation initiation factor 4A2 antibody; IF4A2_HUMAN antibody; N-terminally processed antibody;

eIF4A2 Antibody (YA6850) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using eIF4A2 Antibody (HY-P87157) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • eIF4A2 Antibody (YA6850) Product Image
    1/16
  • Experimental Validation Results for eIF4A2 Antibody (YA6850)
  • eIF4A2 Antibody (YA6850) Product Image
  • Experimental Validation Results for eIF4A2 Antibody (YA6850)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using eIF4A2 Antibody (HY-P87157) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for eIF4A2 Antibody (YA6850)
  • eIF4A2 Antibody (YA6850) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using eIF4A2 Antibody (HY-P87157) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • eIF4A2 Antibody (YA6850) Product Image WB