TIP60 Antibody (YA5082)
(Synonyms: KAT5; HTATIP; TIP60; Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA; 2; -interacting protein)TIP60 Antibody (YA5082) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TIP60.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
WB, FC
-
Reactivity :
Human
-
Formulation:
Supplied in purified antibody in PBS with 0.05% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
FC
FC: Flow Cytometry
|
|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 |
Product Details
TIP60 Antibody (YA5082) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TIP60.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 59 kDa
Purified recombinant fragment of human TIP60 expressed in E. Coli. The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in purified antibody in PBS with 0.05% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Histone acetyltransferase TIP60 (KAT5) is a member of the MYST family of lysine acetyltransferases and functions as the catalytic core of the evolutionarily conserved NuA4 chromatin-modifying complex, where it promotes histone acetylation and transcriptional regulation[1][2]. Mechanistically, TIP60 plays a central role in the DNA damage response by interacting with and acetylating key DNA repair regulators, including ataxia-telangiectasia mutated (ATM), thereby facilitating ATM activation following DNA double-strand breaks[1][3][4]. Following DNA damage, the NuA4/TIP60 complex is recruited to chromatin surrounding DNA lesions and contributes to chromatin remodeling events that support efficient DNA repair[1][5]. TIP60 also regulates the acetylation of multiple non-histone proteins involved in genome maintenance, highlighting its broad role in preserving cell viability and genomic stability[2]. In disease contexts, reduced TIP60 activity or expression has been associated with genomic instability, and experimental evidence supports a tumor-suppressive role for TIP60 in breast cancer models[6]. Beyond cancer, genetic studies demonstrate that TIP60 is required for neural stem cell maintenance, neurogenesis, and normal brain development, indicating functions that extend beyond canonical DNA repair pathways[7]. Compared with related MYST acetyltransferases, TIP60 is distinguished by its direct coupling of chromatin sensing and ATM-dependent DNA damage signaling, making it a widely used experimental model for investigating chromatin-regulated genome surveillance mechanisms[1][3][4].
-
Subcellular Localization
Nucleus; Chromosome; Cytoplasm; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle pole; Nucleus, nucleolus; Cytoplasm, perinuclear region; Cytoplasm
-
Isoforms & Post-Translational Modification
Q92993 has 4 isomers: Q92993-1: 58582 Da (predicted); Q92993-2: 53077 Da (predicted); Q92993-3: 61798 Da (predicted); Q92993-4: 56292 Da (predicted).
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase (PubMed:12468530, PubMed:26829474, PubMed:29335245). The phosphorylated form has a higher activity (PubMed:12468530, PubMed:29335245). Phosphorylation at Ser-90 by CDK1 or CDK9 is a prerequisite for phosphorylation at Ser-86 by GSK3 (PubMed:26829474, PubMed:30704899). Phosphorylation at Ser-86 by GSK3 (GSK3A or GSK3B) activates acetyltransferase and acyltransferase activities (PubMed:30704899). Phosphorylation at Ser-90 by CDK9 promotes KAT5 recruitment to chromatin (PubMed:29335245). Phosphorylation by VRK1 following DNA damage promotes KAT5 association with chromatin and histone acetyltransferase activity (PubMed:33076429);Autoacetylated (PubMed:20100829, PubMed:24835996, PubMed:25301942, PubMed:26291311, PubMed:33938178). Autoacetylation is required for histone acetyltransferase activity (PubMed:20100829, PubMed:25865756). Autoacetylation at Lys-327 is facilitated by interaction with EP300/p300: it prevents ubiquitination and subsequent degradation by the proteasome and promotes acetylation of target proteins (PubMed:24835996). Deacetylated by HDAC3 and SIRT1 (PubMed:20100829, PubMed:25301942). Deacetylation by HDAC3 promotes its ubiquitination and cytoplasmic localization (PubMed:25301942);Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies (PubMed:17704809). Sumoylation with SUMO2 by PIAS4 at Lys-430 promotes repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:32832608). Sumoylation by PIAS4 impairs interaction with PRKDC, inhibiting non-homologous end joining (NHEJ)-mediated repair of DSBs, thereby facilitating HR (PubMed:32832608). Desumoylated by SENP3 (PubMed:32832608);Ubiquitinated by MDM2, leading to its proteasome-dependent degradation (PubMed:11927554, PubMed:24835996). Ubiquitination is prevented by autoacetylation at Lys-327 (PubMed:24835996). Ubiquitinated following deacetylation by HDAC3, leading to cytoplasmic localization (PubMed:25301942). Deubiquitinated by USP7 following interaction with ATF3, promoting its stabilization (PubMed:25865756);(Microbial infection) In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation -
Subunit
Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6.
-
SwissProt ID
-
Synonyms
KAT5; HTATIP; TIP60; Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA; 2; -interacting protein
Documentation
[1]. Squatrito M, et al. Tip60 in DNA damage response and growth control: many tricks in one HAT. Trends Cell Biol. 2006 Sep;16(9):433-42. [Content Brief]
[2]. Sun Y, et al. Tip60: connecting chromatin to DNA damage signaling. Cell Cycle. 2010 Mar 1;9(5):930-6. [Content Brief]
[3]. KAT5 phosphorylation links chromatin to ATM activation. Cancer Discov. 2013 Jul;3(7):OF18. [Content Brief]
[4]. TIP60 gene information from NCBI.
[5]. Liu J, et al. Metabolic modulation of chromatin: implications for DNA repair and genomic integrity. Front Genet. 2013 Sep 17;4:182. [Content Brief]
[6]. Bassi C, et al. The acetyltransferase Tip60 contributes to mammary tumorigenesis by modulating DNA repair. Cell Death Differ. 2016 Jul;23(7):1198-208. [Content Brief]
[7]. Tominaga K, et al. Tip60/KAT5 Histone Acetyltransferase Is Required for Maintenance and Neurogenesis of Embryonic Neural Stem Cells. Int J Mol Sci. 2023 Jan 20;24(3):2113. [Content Brief]
Powered by Bioz
