XRCC1 Antibody (YA1058)
(Synonyms: XRCC1; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1)XRCC1 Antibody (YA1058) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to XRCC1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
XRCC1 Antibody (YA1058) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to XRCC1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 82 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 69 kDa
Purified recombinant human XRCC1 protein fragments expressed in E.coli.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
XRCC1 is a Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity[1][2][3][4][5].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Tissue_specificity:It is expressed (at the protein level) in fibroblasts, retinal pigment epithelial cells, and lymphoblasts. -
Subunit
Homodimer (PubMed:16397295). Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3) (PubMed:19155274, PubMed:19336415). Interacts with APTX and APLF (PubMed:14755728, PubMed:15044383, PubMed:15380105, PubMed:17353262, PubMed:17507382). Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1 (PubMed:19934257). Interacts with (poly-ADP-ribosylated) PARP1 (PubMed:34811483)
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SwissProt ID
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Synonyms
XRCC1; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Whitehouse CJ, et al. XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair. Cell. 2001 Jan 12;104(1):107-17. [Content Brief]
[2]. Hoch NC, et al. XRCC1 mutation is associated with PARP1 hyperactivation and cerebellar ataxia. Nature. 2017 Jan 5;541(7635):87-91. [Content Brief]
[3]. Demin AA, et al. XRCC1 prevents toxic PARP1 trapping during DNA base excision repair. Mol Cell. 2021 Jul 15;81(14):3018-3030.e5. [Content Brief]
[4]. Adamowicz M, et al. XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair. Nat Cell Biol. 2021 Dec;23(12):1287-1298. [Content Brief]
[5]. El-Khamisy SF, et al. A requirement for PARP-1 for the assembly or stability of XRCC1 nuclear foci at sites of oxidative DNA damage. Nucleic Acids Res. 2003 Oct 1;31(19):5526-33. [Content Brief]
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