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Prosurfactant Protein C Antibody (YA6950)

(Synonyms: BRICD6 antibody; BRICHOS domain containing 6 antibody; PSP C antibody; PSPC antibody; PSPC_HUMAN antibody; Pulmonary surfactant apoprotein 2 antibody; Pulmonary surfactant apoprotein PSP C antibody; pulmonary surfactant apoprotein-2 SP-C antibody; Pulmonary surfactant associated protein C antibody; Pulmonary surfactant associated proteolipid SPL pVal antibody)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

Prosurfactant Protein C Antibody (YA6950) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prosurfactant Protein C.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Prosurfactant Protein C Antibody (YA6950) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:5000 1:3000 1:2000 1:1000 1:1000

Product Details

Description

Prosurfactant Protein C Antibody (YA6950) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prosurfactant Protein C.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Immunogen

Synthetic peptide within human Prosurfactant Protein C aa 1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719298

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    Western blot analysis of extracts from Human lung (lane 1(40μg)) 、Mouse lung (lane 2(40μg)) and Rat lung (lane 3(40μg)) using Prosurfactant Protein C Antibody (HY-P87267) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    Western blot analysis was performed on protein extracts from Mouse lung (lane 2, 20 μg), Mouse lung (lane 3, 40 μg), Rat lung (lane 4, 20 μg), and Rat lung (lane 5, 40 μg) using Prosurfactant Protein C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    Prosurfactant Protein C is a Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces

  • Subcellular Localization

    Secreted, extracellular space, surface film

  • Isoforms & Post-Translational Modification

    P11686 has 2 isomers: P11686-1: 21013 Da (predicted); P11686-2: 20321 Da (predicted).

  • SwissProt ID

    P11686

  • Synonyms

    BRICD6 antibody; BRICHOS domain containing 6 antibody; PSP C antibody; PSPC antibody; PSPC_HUMAN antibody; Pulmonary surfactant apoprotein 2 antibody; Pulmonary surfactant apoprotein PSP C antibody; pulmonary surfactant apoprotein-2 SP-C antibody; Pulmonary surfactant associated protein C antibody; Pulmonary surfactant associated proteolipid SPL pVal antibody

Prosurfactant Protein C Antibody (YA6950) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Human lung (lane 1(40μg)) 、Mouse lung (lane 2(40μg)) and Rat lung (lane 3(40μg)) using Prosurfactant Protein C Antibody (HY-P87267) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Mouse lung (lane 2, 20 μg), Mouse lung (lane 3, 40 μg), Rat lung (lane 4, 20 μg), and Rat lung (lane 5, 40 μg) using Prosurfactant Protein C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Prosurfactant Protein C Antibody (YA6950) Product Image
    1/16
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Prosurfactant Protein C Antibody (YA6950) Product Image
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Human lung (lane 1(40μg)) 、Mouse lung (lane 2(40μg)) and Rat lung (lane 3(40μg)) using Prosurfactant Protein C Antibody (HY-P87267) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Mouse lung (lane 2, 20 μg), Mouse lung (lane 3, 40 μg), Rat lung (lane 4, 20 μg), and Rat lung (lane 5, 40 μg) using Prosurfactant Protein C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Experimental Validation Results for Prosurfactant Protein C Antibody (YA6950)
  • Prosurfactant Protein C Antibody (YA6950) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Human lung (lane 1(40μg)) 、Mouse lung (lane 2(40μg)) and Rat lung (lane 3(40μg)) using Prosurfactant Protein C Antibody (HY-P87267) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Prosurfactant Protein C Antibody (YA6950) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts from Mouse lung (lane 2, 20 μg), Mouse lung (lane 3, 40 μg), Rat lung (lane 4, 20 μg), and Rat lung (lane 5, 40 μg) using Prosurfactant Protein C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Prosurfactant Protein C Antibody (YA6950) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Prosurfactant Protein C Antibody (YA6950) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Prosurfactant Protein C Antibody (YA6950) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Prosurfactant Protein C Antibody (YA6950) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinoma tissue using Prosurfactant Protein C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87267, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Prosurfactant Protein C Antibody (YA6950) Product Image WB
  • Prosurfactant Protein C Antibody (YA6950) Product Image WB
  • Prosurfactant Protein C Antibody (YA6950) Product Image IHC-P
  • Prosurfactant Protein C Antibody (YA6950) Product Image IHC-P
  • Prosurfactant Protein C Antibody (YA6950) Product Image mIHC
  • Prosurfactant Protein C Antibody (YA6950) Product Image mIHC