Klotho Antibody(YA6870)

Cat. No.: HY-P87179: Data Sheet User Guide for Antibodies
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Klotho Antibody(YA6870) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Klotho.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Klotho Antibody(YA6870) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Klotho.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 62,116 kDa;

Observed band size: 62,116 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9UEF7

Gene ID

9365 [NCBI]

Immunogen

Synthetic peptide within Human Klotho aa 404-453 / 1,012.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity); The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Apical cell membrane; Single-pass type I membrane protein; Secreted; Secreted

Expression：
Tissue_specificity:It is present in the renal cortical tubules (protein level) . Soluble peptides are present in serum and cerebrospinal fluid. It is expressed in the kidney, placenta, small intestine, and prostate. Its expression is downregulated in the kidneys of renal cell carcinoma, hepatocellular carcinoma, and chronic renal failure.
Positive sample: Human kidney tissue lysates, Human serum lysate, human kidney tissue lysate, human kidney tissue, mouse kidney tissue.

Isoforms & Post-Translational Modification：Q9UEF7 has 2 isomers: Q9UEF7-1: 116181 Da (predicted); Q9UEF7-2: 62135 Da (predicted).
N-glycosylated

Subunit：Homodimer. Interacts with FGF23 and FGFR1

Database

SwissProt: Q9UEF7 Human ; O35082 Mouse

Synonyms

Kl; Klotho peptide

Documentation

User Guide for Antibodies (1077 KB)