2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. ERp57 Antibody (YA3300)

ERp57 Antibody (YA3300) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERp57.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • Beschreibung

Beschreibung

ERp57 Antibody (YA3300) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERp57.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 57 kDa;
Observed band size: 57 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human ERp57 aa150-200.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:50
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HepG2 (lane 2(20μg) and HepG2 (lane 3(40μg) using ERp57 (HY-P83555) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed:11825568, PubMed:16193070, PubMed:27897272, PubMed:36104323, PubMed:7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed:35948544, PubMed:36104323)
Subcellular Localization：Endoplasmic reticulum; Endoplasmic reticulum lumen; Melanosome
Expression：
Tissue_specificity:Detected in the flagellum and head region of spermatozoa (at protein level) (PubMed:20400973) . Expressed in liver, stomach and colon (at protein level) . Expressed in gastric parietal cells and chief cells (at protein level) (PubMed:24188822)
Subunit：Part of the major histocompatibility complex class I (MHC I) peptide loading complex composed of TAP1, TAP2, B2M, MHC heavy chain, TAPBP, PDIA3, and CALR (PubMed:19119025, PubMed:35948544, PubMed:36104323).
RRID
Database

Entrez Gene: 2923 Human

SwissProt: P30101 Human

OMIM: 602046 Human

Research Field

Signal Transduction
Synonyms
ERp57; ERp60; ERp61; Glucose Regulated Protein 58 Kd; HsT17083; p58; PDIA3; Phospholipase C alpha; PI PLC
Dokumentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

ERp57 Antibody (YA3300) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ERp57 Antibody (YA3300)
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SAFETY DATA SHEET (SDS)

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