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ADAMTS1 Antibody (YA4559)

(Synonyms: C3-C5; METH1)
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Based on 1 Customer Validation

ADAMTS1 Antibody (YA4559) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ADAMTS1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • ADAMTS1 Antibody (YA4559) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

ADAMTS1 Antibody (YA4559) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ADAMTS1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70-90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 105 kDa
Immunogen

Purified recombinant fragment of human ADAMTS1 (AA: 858-960) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718991

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
    Western blot analysis of extracts from SK-OV-3 (lane 2(20μg), Hela (lane 3(20μg), HUVEC (lane 4(20μg) and K562 (lane 5(20μg) using ADAMTS1 Antibody (HY-P84862) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
    Western blot analysis of extracts from Hela(lane 1(40μg) ) using ADAMTS1 antibody(HY-P84862). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
    Flow cytometric analysis of 1X10^6 Hela cells labeling ADAMTS1 Antibody(HY-P84862

Background

  • Function

    ADAMTS1 is a Metalloprotease which cleaves aggrecan, a cartilage proteoglycan, at the '1938-Glu-|-Leu-1939' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Also cleaves COMP. Has angiogenic inhibitor activity. May play a critical role in follicular rupture[1][2].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • SwissProt ID

    Q9UHI8

  • Gene ID
    9510 [NCBI]
  • Synonyms

    C3-C5; METH1

ADAMTS1 Antibody (YA4559) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SK-OV-3 (lane 2(20μg), Hela (lane 3(20μg), HUVEC (lane 4(20μg) and K562 (lane 5(20μg) using ADAMTS1 Antibody (HY-P84862) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 1(40μg) ) using ADAMTS1 antibody(HY-P84862). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ADAMTS1 Antibody(HY-P84862

  • ADAMTS1 Antibody (YA4559) Product Image
    1/16
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • ADAMTS1 Antibody (YA4559) Product Image
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SK-OV-3 (lane 2(20μg), Hela (lane 3(20μg), HUVEC (lane 4(20μg) and K562 (lane 5(20μg) using ADAMTS1 Antibody (HY-P84862) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 1(40μg) ) using ADAMTS1 antibody(HY-P84862). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ADAMTS1 Antibody(HY-P84862
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • Experimental Validation Results for ADAMTS1 Antibody (YA4559)
  • ADAMTS1 Antibody (YA4559) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SK-OV-3 (lane 2(20μg), Hela (lane 3(20μg), HUVEC (lane 4(20μg) and K562 (lane 5(20μg) using ADAMTS1 Antibody (HY-P84862) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ADAMTS1 Antibody (YA4559) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 1(40μg) ) using ADAMTS1 antibody(HY-P84862). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • ADAMTS1 Antibody (YA4559) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling ADAMTS1 Antibody(HY-P84862

  • ADAMTS1 Antibody (YA4559) Product Image WB
  • ADAMTS1 Antibody (YA4559) Product Image WB
  • ADAMTS1 Antibody (YA4559) Product Image FC