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Toll-Like Receptor 2 Antibody (YA1932)(PBS only)

(Synonyms: Toll-Like Receptor 2; TLR2; CD282; TIL4; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4)

Toll-Like Receptor 2 Antibody (YA1932) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

Toll-Like Receptor 2 Antibody (YA1932) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 2.

  • Host Rabbit
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 89 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 89 kDa
Immunogen

Recombinant protein of mouse TLR2

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Toll-Like Receptor 2 Antibody (HY-P82187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
    Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
    Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Toll-Like Receptor 2 is a Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides. Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling. Acts as the major receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR1 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen. Recombinant MPT83 from M.tuberculosis stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium. Lung macrophages which express low levels of TLR2 respond poorly to stimulation by M.tuberculosis LpqH. Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM. Interacts with TICAM2; (Microbial infection) Mediates activation of bone marrow-derived dendritic cells and macrophages, and production of pro-inflammatory cytokines, such as IL12 (IL12B/IL12A), triggered by Toxoplasma gondii micronemal protein 4 (MIC4) and micronemal protein 1 (MIC1)[1][2][3][4][5].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein; Membrane raft

  • Expression


    Tissue_specificity:This protein was detected in macrophage lines, smooth muscle, lung, spleen, thymus, brain, and adipose tissue. Cell surface expression was detected in alveolar macrophages and dendritic macrophages, with lower expression levels (protein level) in lung macrophages (PubMed:19362712) .

  • Subunit

    Interacts with LY96, TLR1 and TLR6 (via extracellular domain). TLR2 seems to exist in heterodimers with either TLR1 or TLR6 before stimulation by the ligand (PubMed:19931471). The heterodimers form bigger oligomers in response to their corresponding ligands as well as further heterotypic associations with other receptors such as CD14 and/or CD36 (By similarity). Binds MYD88 (via TIR domain). Interacts with TICAM1 (By similarity). Interacts with CNPY3 (PubMed:18780723). Interacts with ATG16L1 (By similarity). Interacts with non-modified M.tuberculosis protein MPT83 (PubMed:22174456). Interacts with PPP1R11 (PubMed:27805901). Interacts with TIRAP (By similarity)

  • SwissProt ID

    Q9QUN7

  • Gene ID
    24088 [NCBI]
  • Synonyms

    Toll-Like Receptor 2; TLR2; CD282; TIL4; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

  • Research Field

    Immunology

Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Toll-Like Receptor 2 Antibody (HY-P82187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Toll-Like Receptor 2 Antibody (HY-P82187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Experimental Validation Results for Toll-Like Receptor 2 Antibody (YA1932)(PBS only)
  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Toll-Like Receptor 2 Antibody (HY-P82187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling Toll-Like Receptor 2 with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Toll-Like Receptor 2 Antibody (HY-P82187) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image WB
  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image ICC
  • Toll-Like Receptor 2 Antibody (YA1932)(PBS only) Product Image ICC