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Guanylyl Cyclase beta 1 Antibody (YA2147)

(Synonyms: GCbeta1; GCS beta 1; GCS beta 3; GUC1B3; GUCSB3; GUCY1B1; Gucy1b1; GUCY1B3; SGC)
Customer Review

Based on 1 Customer Validation

Guanylyl Cyclase beta 1 Antibody (YA2147) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Guanylyl Cyclase beta 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Guanylyl Cyclase beta 1 Antibody (YA2147) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Guanylyl Cyclase beta 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 71 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 71 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Guanylyl Cyclase beta 1 aa1-64.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104511

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), 293 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using Guanylyl Cyclase beta 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    Guanylyl Cyclase beta 1 mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Detected in brain cortex and cerebellum (at protein level)

  • Isoforms & Post-Translational Modification

    Q02153 has 3 isomers: Q02153-1: 70514 Da (predicted); Q02153-2: 66738 Da (predicted); Q02153-3: 68165 Da (predicted).

  • Subunit

    The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Heterodimer with GUCY1A1 (PubMed:1352257, PubMed:23505436, PubMed:24669844). Can also form inactive homodimers in vitro (PubMed:23505436, PubMed:24669844)

  • SwissProt ID

    Q02153

  • Gene ID
    2983 [NCBI]
  • Synonyms

    GCbeta1; GCS beta 1; GCS beta 3; GUC1B3; GUCSB3; GUCY1B1; Gucy1b1; GUCY1B3; SGC

  • Research Field

    Cell Biology

Guanylyl Cyclase beta 1 Antibody (YA2147) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), 293 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using Guanylyl Cyclase beta 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    1/16
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), 293 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using Guanylyl Cyclase beta 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Experimental Validation Results for Guanylyl Cyclase beta 1 Antibody (YA2147)
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), 293 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using Guanylyl Cyclase beta 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Guanylyl Cyclase beta 1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82402, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image WB
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P
  • Guanylyl Cyclase beta 1 Antibody (YA2147) Product Image IHC-P