Copy Link Print Information

Guanylyl Cyclase alpha 1 Antibody (YA2937)

(Synonyms: GUCY1A3; GC-S-alpha-1; GUC1A3; GUCA3; GCS-alpha-3; GUCY1A1; GC-SA3; GCS-alpha-1; GUCSA3)
Customer Review

Based on 1 Customer Validation

Guanylyl Cyclase alpha 1 Antibody (YA2937) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Guanylyl Cyclase alpha 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Guanylyl Cyclase alpha 1 Antibody (YA2937)
  • Guanylyl Cyclase alpha 1 Antibody (YA2937) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Guanylyl Cyclase alpha 1 Antibody (YA2937) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Guanylyl Cyclase alpha 1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 73 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 77 kDa
Species Reactivity Database

Entrez Gene: 2982 Human

SwissProt: Q02108 Human

Immunogen

A synthesized peptide derived from human GCYA3 aa1-186.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105307

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Guanylyl Cyclase alpha 1 Antibody (YA2937)
    Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Guanylyl Cyclase alpha 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Detected in the cerebral cortex and lungs (protein level)

  • Isoforms & Post-Translational Modification

    Q02108 has 2 isomers: Q02108-1: 77452 Da (predicted); Q02108-2: 70184 Da (predicted).

  • Subunit

    The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Heterodimer with GUCY1B1 (PubMed:23505436, PubMed:24669844, PubMed:9742212)

  • SwissProt ID

    Q02108

  • Gene ID
    2982 [NCBI]
  • Synonyms

    GUCY1A3; GC-S-alpha-1; GUC1A3; GUCA3; GCS-alpha-3; GUCY1A1; GC-SA3; GCS-alpha-1; GUCSA3

  • Research Field

    Signal Transduction

Guanylyl Cyclase alpha 1 Antibody (YA2937) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Guanylyl Cyclase alpha 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Guanylyl Cyclase alpha 1 Antibody (YA2937) Product Image
    1/16
  • Experimental Validation Results for Guanylyl Cyclase alpha 1 Antibody (YA2937)
  • Guanylyl Cyclase alpha 1 Antibody (YA2937) Product Image
  • Experimental Validation Results for Guanylyl Cyclase alpha 1 Antibody (YA2937)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Guanylyl Cyclase alpha 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Guanylyl Cyclase alpha 1 Antibody (YA2937)
  • Guanylyl Cyclase alpha 1 Antibody (YA2937) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Guanylyl Cyclase alpha 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Guanylyl Cyclase alpha 1 Antibody (YA2937) Product Image WB