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ORP1 Antibody (YA1477)

(Synonyms: ORP1; OSBP8; OSBPL1; OSBPL1B)
Customer Review

Based on 1 Customer Validation

ORP1 Antibody (YA1477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ORP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • ORP1 Antibody (YA1477) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:100-1:200 1:10-1:100

Product Details

Description

ORP1 Antibody (YA1477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ORP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 109,50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 108 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human ORP1 aa937-950.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ORP1 Antibody (YA1477)
    Western blot analysis was performed on extracts from NCI-H1299 (lane 1, 15 μg), Hela (lane 2, 15 μg), C6 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using ORP1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ORP1 Antibody (YA1477)
    Western blot analysis of extracts from A549 (lane 2(20μg), HEK293T (lane 3(20μg), U87 (lane 4(20μg) and Hela (lane 5(20μg) using ORP1 Antibody (HY-P81732) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ORP1 Antibody (YA1477)
    Immunocytochemistry analysis of U87 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ORP1 Antibody (YA1477)
    Immunocytochemistry analysis of A549 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ORP1 binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Binds 25-hydroxycholesterol and cholesterol[1][2].

  • Subcellular Localization

    Late endosome

  • Isoforms & Post-Translational Modification

    Q9BXW6 has 3 isomers: Q9BXW6-1: 108470 Da (predicted); Q9BXW6-2: 50253 Da (predicted); Q9BXW6-4: 65095 Da (predicted).

  • Subunit

    Interacts (via FFAT motif) with VAPA and VAPB (PubMed:33124732). Interacts with the GTP-bound form of RAB7A (PubMed:17081983).

  • SwissProt ID

    Q9BXW6

  • Gene ID
    114876 [NCBI]
  • Synonyms

    ORP1; OSBP8; OSBPL1; OSBPL1B

  • Research Field

    Signal Transduction

ORP1 Antibody (YA1477) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from NCI-H1299 (lane 1, 15 μg), Hela (lane 2, 15 μg), C6 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using ORP1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HEK293T (lane 3(20μg), U87 (lane 4(20μg) and Hela (lane 5(20μg) using ORP1 Antibody (HY-P81732) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U87 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ORP1 Antibody (YA1477) Product Image
    1/16
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • ORP1 Antibody (YA1477) Product Image
  • Experimental Validation Results for ORP1 Antibody (YA1477)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from NCI-H1299 (lane 1, 15 μg), Hela (lane 2, 15 μg), C6 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using ORP1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ORP1 Antibody (YA1477)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HEK293T (lane 3(20μg), U87 (lane 4(20μg) and Hela (lane 5(20μg) using ORP1 Antibody (HY-P81732) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ORP1 Antibody (YA1477)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U87 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ORP1 Antibody (YA1477)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • Experimental Validation Results for ORP1 Antibody (YA1477)
  • ORP1 Antibody (YA1477) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from NCI-H1299 (lane 1, 15 μg), Hela (lane 2, 15 μg), C6 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using ORP1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ORP1 Antibody (YA1477) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2(20μg), HEK293T (lane 3(20μg), U87 (lane 4(20μg) and Hela (lane 5(20μg) using ORP1 Antibody (HY-P81732) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ORP1 Antibody (YA1477) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U87 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ORP1 Antibody (YA1477) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ORP1 Antibody (YA1477) Product Image WB
  • ORP1 Antibody (YA1477) Product Image WB
  • ORP1 Antibody (YA1477) Product Image ICC
  • ORP1 Antibody (YA1477) Product Image ICC