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  5. ORP1 Antibody (YA1477)

ORP1 Antibody (YA1477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ORP1.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • 제품 설명

  • References

제품 설명

ORP1 Antibody (YA1477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ORP1.
Host

Rabbit
Clonality

Recombinant,Monoclonal
분자량
Predicted band size: 108 kDa;
Observed band size: 109,50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human ORP1 aa937-950.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:5000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-1:200
FC
FC: Flow Cytometry
1:10-1:100
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
ALL WB ICC

  • Western blot analysis was performed on extracts from NCI-H1299 (lane 1, 15 μg), Hela (lane 2, 15 μg), C6 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using ORP1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis of extracts from A549 (lane 2(20μg), HEK293T (lane 3(20μg), U87 (lane 4(20μg) and Hela (lane 5(20μg) using ORP1 Antibody (HY-P81732) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of U87 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of A549 cells labeling ORP1 with ORP1 Antibody (HY-P81732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ORP1 Antibody (HY-P81732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function：Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (By similarity). Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A (PubMed:16176980). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193)
Subcellular Localization：Late endosome
Isoforms & Post-Translational Modification：Q9BXW6 has 3 isomers: Q9BXW6-1: 108470 Da (predicted); Q9BXW6-2: 50253 Da (predicted); Q9BXW6-4: 65095 Da (predicted).
Subunit：Interacts (via FFAT motif) with VAPA and VAPB (PubMed:33124732). Interacts with the GTP-bound form of RAB7A (PubMed:17081983).
Database

Entrez Gene: 114876 Human ; 64291 Mouse ; 259221 Rat

SwissProt: Q9BXW6 Human ; Q91XL9 Mouse ; Q8K4M9 Rat

OMIM: 606730 Human

Research Field

Signal Transduction
Synonyms
ORP1; OSBP8; OSBPL1; OSBPL1B
각종 서류
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
References

ORP1 Antibody (YA1477) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
ORP1 Antibody (YA1477)
Cat. No.:
HY-P81732
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