CCK-4 Antibody (YA4231)

Cat. No.: HY-P84534: Data Sheet COA
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CCK-4 Antibody (YA4231) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCK-4.

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사이즈	재고	수량
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명
References

제품 설명

CCK-4 Antibody (YA4231) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCK-4.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 118 kDa;

Observed band size: 118 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13308

Gene ID

5754 [NCBI]

Immunogen

Purified recombinant fragment of human PTK7 aa 31-199.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa(lane 2(20μg) , Jurkat(lane 3(20μg) ,HCT116(lane 4(20μg) and 293T( lane 5(20μg) using CCK-4 Antibody (HY-P84534). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from K562(lane 1(40μg) )

Background

Function：Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis

Subcellular Localization：Membrane; Single-pass type I membrane protein; Cell junction

Expression：
Tissue_specificity:It is highly expressed in the lungs, liver, pancreas, kidneys, placenta, and melanocytes. It is weakly expressed in the thyroid, ovaries, brain, heart, and skeletal muscle. It is also expressed in erythroleukemia cells, but not in the colon.

Induction:Higher expression in cell lines established from normal non-tumorigenic tissues compared to cell lines established from highly metastatic invasive carcinomas (at protein level)

Isoforms & Post-Translational Modification：Q13308 has 6 isomers: Q13308-1: 118392 Da (predicted); Q13308-2: 113805 Da (predicted); Q13308-3: 103581 Da (predicted); Q13308-4: 112261 Da (predicted); Q13308-5: 89764 Da (predicted); Q13308-6: 119197 Da (predicted).
MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway

Subunit：Interacts with CTNNB1

RRID

AB_3718928

Synonyms

CCK4; PTK7

각종 서류

Select Batch:

Data Sheet (258 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/a549-cell.html [Content Brief]