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CCK-4 Antibody (YA4231)

(Synonyms: CCK4; PTK7)
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Based on 1 Customer Validation

CCK-4 Antibody (YA4231) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCK-4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
  • CCK-4 Antibody (YA4231) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

CCK-4 Antibody (YA4231) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCK-4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 118 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 118 kDa
Immunogen

Purified recombinant fragment of human PTK7 aa 31-199.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718928

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CCK-4 Antibody (YA4231)
    Western blot analysis of extracts from HeLa(lane 2(20μg) , Jurkat(lane 3(20μg) ,HCT116(lane 4(20μg) and 293T( lane 5(20μg) using CCK-4 Antibody (HY-P84534). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
    Western blot analysis of extracts from K562(lane 1(40μg) )

Background

  • Function

    CCK-4 is an Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein; Cell junction

  • Expression


    Tissue_specificity:It is highly expressed in the lungs, liver, pancreas, kidneys, placenta, and melanocytes. It is weakly expressed in the thyroid, ovaries, brain, heart, and skeletal muscle. It is also expressed in erythroleukemia cells, but not in the colon.

    Induction:Higher expression in cell lines established from normal non-tumorigenic tissues compared to cell lines established from highly metastatic invasive carcinomas (at protein level)

  • Isoforms & Post-Translational Modification

    Q13308 has 6 isomers: Q13308-1: 118392 Da (predicted); Q13308-2: 113805 Da (predicted); Q13308-3: 103581 Da (predicted); Q13308-4: 112261 Da (predicted); Q13308-5: 89764 Da (predicted); Q13308-6: 119197 Da (predicted).
    MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway

  • Subunit

    Interacts with CTNNB1

  • SwissProt ID

    Q13308

  • Gene ID
    5754 [NCBI]
  • Synonyms

    CCK4; PTK7

CCK-4 Antibody (YA4231) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg) , Jurkat(lane 3(20μg) ,HCT116(lane 4(20μg) and 293T( lane 5(20μg) using CCK-4 Antibody (HY-P84534). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 1(40μg) )

  • CCK-4 Antibody (YA4231) Product Image
    1/16
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
  • CCK-4 Antibody (YA4231) Product Image
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg) , Jurkat(lane 3(20μg) ,HCT116(lane 4(20μg) and 293T( lane 5(20μg) using CCK-4 Antibody (HY-P84534). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 1(40μg) )
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
  • Experimental Validation Results for CCK-4 Antibody (YA4231)
  • CCK-4 Antibody (YA4231) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg) , Jurkat(lane 3(20μg) ,HCT116(lane 4(20μg) and 293T( lane 5(20μg) using CCK-4 Antibody (HY-P84534). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • CCK-4 Antibody (YA4231) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 1(40μg) )

  • CCK-4 Antibody (YA4231) Product Image WB
  • CCK-4 Antibody (YA4231) Product Image WB