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ACAT1 Antibody (YA1971)

(Synonyms: T2; MAT; ACAT; THIL)
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Based on 1 Customer Validation

ACAT1 Antibody (YA1971) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACAT1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ACAT1 Antibody (YA1971)
  • ACAT1 Antibody (YA1971) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:1:100 1:20

Product Details

Description

ACAT1 Antibody (YA1971) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACAT1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database

Entrez Gene: 38 Human

SwissProt: P24752 Human

Immunogen

A synthetic peptide of human ACAT1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ACAT1 Antibody (YA1971)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), DU145 (lane 4), and Rat heart (lane 5) using ACAT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    ACAT1, also termed sterol O-acyltransferase 1 (SOAT1), is an endoplasmic reticulum-associated enzyme that catalyzes the conversion of free cholesterol and long-chain fatty acyl-CoA into cholesteryl esters, thereby maintaining intracellular cholesterol homeostasis and promoting lipid droplet formation[1][5]. Mechanistically, ACAT1-mediated cholesterol esterification limits excess free cholesterol accumulation and regulates cholesterol storage, linking membrane cholesterol balance to cellular metabolic adaptation[1][6]. In macrophages, increased cholesterol influx activates ACAT1 and drives cholesteryl ester deposition, a process closely associated with foam-cell formation and early atherosclerotic lesion development[7][2]. Experimental inhibition of ACAT activity enhances the pool of free cholesterol available for efflux and alters macrophage cholesterol metabolism, making ACAT1 a widely studied target in atherosclerosis models[7][2]. Beyond cardiovascular disease, dysregulated ACAT1-dependent cholesterol esterification has been reported in cancer and neurodegenerative disorders, where elevated cholesteryl ester accumulation is linked to altered cellular cholesterol handling[5][1][3]. Compared with the related isoform ACAT2/SOAT2, which is enriched in intestine and liver and participates in lipoprotein-associated cholesteryl ester production, ACAT1 is broadly expressed in peripheral tissues and is the dominant isoform involved in macrophage cholesterol storage and foam-cell biology[6][4]. For experimental applications, inhibitors including avasimibe and the ACAT1-selective compound K-604 have been used to investigate cholesterol esterification, plaque remodeling, and disease-associated lipid metabolic pathways[2][8].

  • Subcellular Localization

    Mitochondrion

  • Isoforms & Post-Translational Modification

    P24752 has 2 isomers: P24752-1: 45200 Da (predicted); P24752-2: 17175 Da (predicted).
    Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity)

  • Subunit

    Homotetramer

  • SwissProt ID

    P24752

  • Gene ID
    38 [NCBI]
  • Synonyms

    T2; MAT; ACAT; THIL

  • Research Field

    Tags & Cell Markers

References

[1]. Bhattacharjee P, et al. Targeting Sterol O-Acyltransferase/Acyl-CoA:Cholesterol Acyltransferase (ACAT): A Perspective on Small-Molecule Inhibitors and Their Therapeutic Potential. J Med Chem. 2022 Dec 22;65(24):16062-16098. [Content Brief]

[2]. Hai Q, et al. Acyl-Coenzyme A: Cholesterol Acyltransferase (ACAT) in Cholesterol Metabolism: From Its Discovery to Clinical Trials and the Genomics Era. Metabolites. 2021;11(8):543. [Content Brief]

[3]. Kiros M, et al. Trends in HIV-1 pretreatment drug resistance and HIV-1 variant dynamics among antiretroviral therapy-naive Ethiopians from 2003 to 2018: a pooled sequence analysis. Virol J. 2023 Oct 25;20(1):243. [Content Brief]

[4]. Ohshiro T. Isoform-specific inhibitors of ACATs: recent advances and promising developments. Future Med Chem. 2011 Dec;3(16):2039-61.

[5]. Liu Q, et al. ACAT1/SOAT1 maintains adipogenic ability in preadipocytes by regulating cholesterol homeostasis. J Lipid Res. 2024;65(12):100680.

[6]. Chang TY, et al. Blocking cholesterol storage to treat Alzheimer’s disease. Explor Neuroprot Ther. 2021;1:173-184.

[7]. Tu T, et al. Targeting sterol-O-acyltransferase 1 to disrupt cholesterol metabolism for cancer therapy. Front Oncol. 2023;13:1197502.

[8]. Bocan TM, et al. The ACAT inhibitor avasimibe reduces macrophages and matrix metalloproteinase expression in atherosclerotic lesions of hypercholesterolemic rabbits. Arterioscler Thromb Vasc Biol. 2000 Jan;20(1):70-9. [Content Brief]

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ACAT1 Antibody (YA1971) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), DU145 (lane 4), and Rat heart (lane 5) using ACAT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ACAT1 Antibody (YA1971) Product Image
    1/16
  • Experimental Validation Results for ACAT1 Antibody (YA1971)
  • ACAT1 Antibody (YA1971) Product Image
  • Experimental Validation Results for ACAT1 Antibody (YA1971)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), DU145 (lane 4), and Rat heart (lane 5) using ACAT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ACAT1 Antibody (YA1971)
  • ACAT1 Antibody (YA1971) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), DU145 (lane 4), and Rat heart (lane 5) using ACAT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ACAT1 Antibody (YA1971) Product Image WB