Phospho-IRF3 (Ser386) Antibody (YA7355)

Cat. No.: HY-P87670: Ficha de datos COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospho-IRF3 (Ser386) Antibody (YA7355) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-IRF3 (Ser386).

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn
Referencias

Descripciòn

Phospho-IRF3 (Ser386) Antibody (YA7355) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-IRF3 (Ser386).

Host

Rabbit

Clonality

Monoclonal,Recombinant

Peso molecular

Predicted band size: 47 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q14653

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:15000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Sensitivity	Endogenous	Pureza	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of HeLa cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of MCF-7 cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:24049179, PubMed:25636800, PubMed:27302953, PubMed:31340999, PubMed:36603579, PubMed:8524823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995, PubMed:36603579, PubMed:8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148, PubMed:36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148)

Subcellular Localization：Cytoplasm,Nucleus,Mitochondrion

Expression：
Tissue_Specificity: Expressed constitutively in a variety of tissues

Isoforms & Post-Translational Modification：Q14653 has 5 isomers: Q14653-1: 47219 Da (predicted); Q14653-2: 33170 Da (predicted); Q14653-3: 16730 Da (predicted); Q14653-4: 49134 Da (predicted); Q14653-5: 12330 Da (predicted).
Constitutively phosphorylated on many Ser/Thr residues (PubMed:22394562, PubMed:23478265, PubMed:23746807)丨Pyrophosphorylated by UAP1 following phosphorylation at Ser-386 by TBK1 (PubMed:36603579)丨Acetylation at Lys-366 by KAT8 inhibits recruimtent to promoters and transcription factor activity丨Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation (PubMed:18711448)丨ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding丨Proteolytically cleaved by apoptotic caspases during apoptosis, leading to its inactivation (PubMed:30878284)丨(Microbial infection) ISGylated丨(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3丨(Microbial infection) Phosphorylated by herpes simplex virus 1/HHV-1 US3 at Ser-175 to prevent IRF3 activation

Subunit：Monomer (PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:36603579)

Documentación

Select Batch:

Ficha de datos (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/bxpc-3-cell.html [Content Brief]