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Phospho-IRF3 (Ser386) Antibody (YA7355)

(Synonyms: Interferon regulatory factor 3, IRF-3, IRF3)
Customer Review

Based on 1 Customer Validation

Phospho-IRF3 (Ser386) Antibody (YA7355) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-IRF3 (Ser386).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
  • Phospho-IRF3 (Ser386) Antibody (YA7355) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:1000-1:15000 1:200-1:1000 1:5000-1:20000

Product Details

Description

Phospho-IRF3 (Ser386) Antibody (YA7355) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-IRF3 (Ser386).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
    Immunocytochemistry analysis of HeLa cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
    Immunocytochemistry analysis of MCF-7 cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    IRF3 is key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis. Key transcription factor regulating the IFN response during SARS-CoV-2 infection[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].

  • Subcellular Localization

    Cytoplasm,Nucleus,Mitochondrion

  • Expression


    Tissue_Specificity: Expressed constitutively in a variety of tissues

  • Isoforms & Post-Translational Modification

    Q14653 has 5 isomers: Q14653-1: 47219 Da (predicted); Q14653-2: 33170 Da (predicted); Q14653-3: 16730 Da (predicted); Q14653-4: 49134 Da (predicted); Q14653-5: 12330 Da (predicted).
    Constitutively phosphorylated on many Ser/Thr residues (PubMed:22394562, PubMed:23478265, PubMed:23746807)丨Pyrophosphorylated by UAP1 following phosphorylation at Ser-386 by TBK1 (PubMed:36603579)丨Acetylation at Lys-366 by KAT8 inhibits recruimtent to promoters and transcription factor activity丨Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation (PubMed:18711448)丨ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding丨Proteolytically cleaved by apoptotic caspases during apoptosis, leading to its inactivation (PubMed:30878284)丨(Microbial infection) ISGylated丨(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3丨(Microbial infection) Phosphorylated by herpes simplex virus 1/HHV-1 US3 at Ser-175 to prevent IRF3 activation

  • Subunit

    Monomer (PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:36603579)

  • SwissProt ID

    Q14653

  • Synonyms

    Interferon regulatory factor 3, IRF-3, IRF3

Phospho-IRF3 (Ser386) Antibody (YA7355) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-IRF3 (Ser386) Antibody (YA7355) Product Image
    1/16
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
  • Phospho-IRF3 (Ser386) Antibody (YA7355) Product Image
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
  • Experimental Validation Results for Phospho-IRF3 (Ser386) Antibody (YA7355)
  • Phospho-IRF3 (Ser386) Antibody (YA7355) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-IRF3 (Ser386) Antibody (YA7355) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Phospho-IRF3 (Ser386) with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IRF3 (Ser386) Antibody (HY-P87670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-IRF3 (Ser386) Antibody (YA7355) Product Image ICC
  • Phospho-IRF3 (Ser386) Antibody (YA7355) Product Image ICC