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Rab10 Antibody (YA4117)

(Synonyms: RAB10)
Customer Review

Based on 1 Customer Validation

Rab10 Antibody (YA4117) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Rab10.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Rab10 Antibody (YA4117) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

Rab10 Antibody (YA4117) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Rab10.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Immunogen

Purified recombinant fragment of human Rab10 aa 106-186.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Rab10 Antibody (YA4117)
    Western blot analysis of extracts from HeLa(lane 2(40μg),A549(lane 3(40μg), MCF7(lane 4(40μg),HCT 116(lane 5(40μg)using Rab10 Antibody (YA4117). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem
  • Experimental Validation Results for Rab10 Antibody (YA4117)
    Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Rab10 Antibody (YA4117)
    Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Rab10 is a The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Also plays a specific role in asymmetric protein transport to the plasma membrane. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane. In epithelial cells, it regulates transport from the Golgi to the basolateral membrane. May play a role in the basolateral recycling pathway and in phagosome maturation. May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis. When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route. Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins; (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side; Golgi apparatus membrane; Golgi apparatus, trans-Golgi network membrane; Endosome membrane; Recycling endosome membrane; Cytoplasmic vesicle, phagosome membrane; Cytoplasm, cytoskeleton, cilium basal body; Endoplasmic reticulum membrane; Cytoplasm, perinuclear region; Lysosome

  • Expression


    Tissue_specificity:Expression in hippocampus (PubMed:29562525) . Expression in neutrophils (protein level) (PubMed:29127255) . Expression in testes (protein level) (PubMed:28067790) .

  • Subunit

    Interacts with MYO5A; mediates the transport to the plasma membrane of SLC2A4/GLUT4 storage vesicles (PubMed:22908308).

  • SwissProt ID

    P61026

  • Gene ID
    10890 [NCBI]
  • Synonyms

    RAB10

Rab10 Antibody (YA4117) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(40μg),A549(lane 3(40μg), MCF7(lane 4(40μg),HCT 116(lane 5(40μg)using Rab10 Antibody (YA4117). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Rab10 Antibody (YA4117) Product Image
    1/16
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Rab10 Antibody (YA4117) Product Image
  • Experimental Validation Results for Rab10 Antibody (YA4117)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(40μg),A549(lane 3(40μg), MCF7(lane 4(40μg),HCT 116(lane 5(40μg)using Rab10 Antibody (YA4117). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem
  • Experimental Validation Results for Rab10 Antibody (YA4117)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Rab10 Antibody (YA4117)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Experimental Validation Results for Rab10 Antibody (YA4117)
  • Rab10 Antibody (YA4117) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(40μg),A549(lane 3(40μg), MCF7(lane 4(40μg),HCT 116(lane 5(40μg)using Rab10 Antibody (YA4117). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • Rab10 Antibody (YA4117) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Rab10 Antibody (YA4117) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Rab10 with Rab10 Antibody (HY-P84420) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Rab10 Antibody (HY-P84420) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Rab10 Antibody (YA4117) Product Image WB
  • Rab10 Antibody (YA4117) Product Image ICC
  • Rab10 Antibody (YA4117) Product Image ICC