TRPV4 Antibody
(Synonyms: VRL2, VROAC, TRPV4, Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, Transient receptor potential protein 12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, OTRPC4, TRP12, VRL-2, VR-OAC)TRPV4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TRPV4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
TRPV4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TRPV4.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 98 kDa
Synthetic peptide corresponding to the center region of human TRPV4.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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선적
Shipping with blue ice.
Background
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Function
TRPV4 is a Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Together with PKD2, forms mechano- and thermosensitive channels in cilium. Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage. In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus. In neurons of the central nervous system, could play a role in triggering voluntary water intake in response to increased sodium concentration in body fluid (By similarity)[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cell membrane; Apical cell membrane; Cell junction, adherens junction; Cell projection, cilium
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Expression
Tissue_Specificity: Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level). -
Isoforms & Post-Translational Modification
TRPV4 has 6 isoforms, Q9HBA0-1: amino acid length is 871, molecular weight is 98281 Da (predicted); Q9HBA0-2: amino acid length is 811, molecular weight is 91261 Da (predicted); Q9HBA0-3: amino acid length is 853, molecular weight is 96449 Da (predicted); Q9HBA0-4: amino acid length is 824, molecular weight is 92904 Da (predicted); Q9HBA0-5: amino acid length is 837, molecular weight is 94998 Da (predicted); Q9HBA0-6: amino acid length is 764, molecular weight is 85884 Da (predicted).可发生 N-糖基化TRPV4 存在 6 个异构体，Q9HBA0-1：氨基酸个数为 871 个，分子量为 98281 Da (预测)；Q9HBA0-2：氨基酸个数为 811 个，分子量为 91261 Da (预测)；Q9HBA0-3：氨基酸个数为 853 个，分子量为 96449 Da (预测)；Q9HBA0-4：氨基酸个数为 824 个，分子量为 92904 Da (预测)；Q9HBA0-5：氨基酸个数为 837 个，分子量为 94998 Da (预测)；Q9HBA0-6：氨基酸个数为 764 个，分子量为 85884 Da (预测)。N-glycosylated
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Subunit
Homotetramer (Probable).
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SwissProt ID
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Synonyms
VRL2, VROAC, TRPV4, Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, Transient receptor potential protein 12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, OTRPC4, TRP12, VRL-2, VR-OAC
각종 서류
[1]. Arniges M, et al. Human TRPV4 channel splice variants revealed a key role of ankyrin domains in multimerization and trafficking. J Biol Chem. 2006 Jan 20;281(3):1580-6. [Content Brief]
[2]. Köttgen M, et al. TRPP2 and TRPV4 form a polymodal sensory channel complex. J Cell Biol. 2008 Aug 11;182(3):437-47. [Content Brief]
[3]. Garcia-Elias A, et al. IP3 receptor binds to and sensitizes TRPV4 channel to osmotic stimuli via a calmodulin-binding site. J Biol Chem. 2008 Nov 14;283(46):31284-8. [Content Brief]
[4]. Fiorillo C, et al. TRPV4 mutations in children with congenital distal spinal muscular atrophy. Neurogenetics. 2012 Aug;13(3):195-203. [Content Brief]
[5]. Thorneloe KS, et al. An orally active TRPV4 channel blocker prevents and resolves pulmonary edema induced by heart failure. Sci Transl Med. 2012 Nov 7;4(159):159ra148. [Content Brief]
[6]. Doñate-Macián P, et al. The TRPV4 channel links calcium influx to DDX3X activity and viral infectivity. Nat Commun. 2018 Jun 13;9(1):2307. [Content Brief]
[7]. Landouré G, et al. Mutations in TRPV4 cause Charcot-Marie-Tooth disease type 2C. Nat Genet. 2010 Feb;42(2):170-4. [Content Brief]
[8]. Lamandé SR, et al. Mutations in TRPV4 cause an inherited arthropathy of hands and feet. Nat Genet. 2011 Oct 2;43(11):1142-6. [Content Brief]
[9]. Takahashi N, et al. TRPV4 channel activity is modulated by direct interaction of the ankyrin domain to PI(4,5)P₂. Nat Commun. 2014 Sep 26;5:4994. [Content Brief]
[10]. Strotmann R, et al. Ca2+-dependent potentiation of the nonselective cation channel TRPV4 is mediated by a C-terminal calmodulin binding site. J Biol Chem. 2003 Jul 18;278(29):26541-9. [Content Brief]
[11]. Itoh Y, et al. An environmental sensor, TRPV4 is a novel regulator of intracellular Ca2+ in human synoviocytes. Am J Physiol Cell Physiol. 2009 Nov;297(5):C1082-90. [Content Brief]
[12]. Hurd L, et al. A mutation in TRPV4 results in altered chondrocyte calcium signaling in severe metatropic dysplasia. Am J Med Genet A. 2015 Oct;167A(10):2286-93. [Content Brief]
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