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SH2D1A Antibody (YA1950)

(Synonyms: LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A)
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Based on 1 Customer Validation

SH2D1A Antibody (YA1950) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SH2D1A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SH2D1A Antibody (YA1950)
  • SH2D1A Antibody (YA1950) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

SH2D1A Antibody (YA1950) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SH2D1A.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 14 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human SH2D1A/SAP

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104318

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SH2D1A Antibody (YA1950)
    Western blot analysis of extracts from NIH3T3 (lane 2(20μg) and NIH3T3 (lane 3(40μg) using SH2D1A (HY-P82205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    SH2D1A is a Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2. However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1. Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T-lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes with rheumatoid arthritis

  • Isoforms & Post-Translational Modification

    O60880 has 6 isomers: O60880-1: 14187 Da (predicted); O60880-2: 8392 Da (predicted); O60880-3: 6284 Da (predicted); O60880-4: 13928 Da (predicted); O60880-5: 8188 Da (predicted); O60880-6: 6631 Da (predicted).

  • Subunit

    Interacts with NTRK1, NTRK2 and NTRK3 (By similarity). Interacts with CD84, CD244, LY9, SLAMF1 and FYN

  • SwissProt ID

    O60880

  • Gene ID
    4068 [NCBI]
  • Synonyms

    LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A

  • Research Field

    Cardiovascular

SH2D1A Antibody (YA1950) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH3T3 (lane 2(20μg) and NIH3T3 (lane 3(40μg) using SH2D1A (HY-P82205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SH2D1A Antibody (YA1950) Product Image
    1/16
  • Experimental Validation Results for SH2D1A Antibody (YA1950)
  • SH2D1A Antibody (YA1950) Product Image
  • Experimental Validation Results for SH2D1A Antibody (YA1950)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH3T3 (lane 2(20μg) and NIH3T3 (lane 3(40μg) using SH2D1A (HY-P82205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SH2D1A Antibody (YA1950)
  • SH2D1A Antibody (YA1950) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH3T3 (lane 2(20μg) and NIH3T3 (lane 3(40μg) using SH2D1A (HY-P82205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SH2D1A Antibody (YA1950) Product Image WB