GNA13 Antibody (YA2878)(PBS only)

Cat. No.: HY-P83133A: Data Sheet User Guide for Antibodies
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GNA13 Antibody (YA2878) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNA13.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GNA13 Antibody (YA2878) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNA13.

Host

Rabbit

Molecular Weight

Predicted band size: 44 kDa;

Observed band size: 44 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q14344

Gene ID

10672 [NCBI]

Immunogen

A synthetic peptide of human G protein alpha 13

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16705036, PubMed:16787920, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:12515866, PubMed:15240885). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16705036, PubMed:16787920, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in a process independent from Rho activation (PubMed:11976333). In lymphoid follicles, transmits P2RY8- and S1PR2-dependent signals that lead to inhibition of germinal center (GC) B cell growth and migration outside the GC niche

Subcellular Localization：Cell membrane; Lipid-anchor; Melanosome; Cytoplasm; Nucleus

Expression：
Tissue_specificity:Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level) . Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile (at protein level)

Isoforms & Post-Translational Modification：Q14344 has 2 isomers: Q14344-1: 44050 Da (predicted); Q14344-2: 33287 Da (predicted).
Palmitoylation is critical for proper membrane localization and signaling;Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation

Subunit：G proteins are composed of 3 units; alpha, beta and gamma (PubMed:12399457). Interacts with UBXD5 (PubMed:16202387). Interacts with HAX1 (PubMed:15339924).

Research Field

Signal Transduction

Synonyms

guanine nucleotide binding protein (G protein); alpha 13; G13

Documentation

User Guide for Antibodies (1077 KB)