ABCB5 Antibody (YA836)(PBS only)

Cat. No.: HY-P80527A: Data Sheet User Guide for Antibodies
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ABCB5 Antibody (YA836) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCB5.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ABCB5 Antibody (YA836) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCB5.

Host

Mouse

Molecular Weight

Predicted band size: 139 kDa;

Observed band size: 90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q2M3G0

Gene ID

340273 [NCBI]

Immunogen

Synthetic peptide corresponding to Human ABCB5.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:12960149, PubMed:15205344, PubMed:15899824, PubMed:22306008). Specifically present in limbal stem cells, where it plays a key role in corneal development and repair (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:In epidermal melanocytes, CD133-expressing progenitor cells express this protein (protein level) . This protein is widely expressed and specifically expressed in pigment cells. It is highly expressed in various malignant tissues: highly expressed in clinical melanoma and lowly expressed in normal skin. In melanoma, this protein marks malignant melanoma initiating cells (MMICs) , which have unlimited self-renewal capacity, leading to irreversible tumor progression and metastasis, thus triggering clinical malignancy. This protein is also highly expressed in various leukemia cells. It is also expressed in the basal limbal epithelium.

Isoforms & Post-Translational Modification：Q2M3G0 has 4 isomers: Q2M3G0-4: 138641 Da (predicted); Q2M3G0-2: 14691 Da (predicted); Q2M3G0-3: 14081 Da (predicted); Q2M3G0-1: 89832 Da (predicted).

Research Field

Signal Transduction

Synonyms

ABCB5 P-gp; ATP binding cassette sub family B (MDR/TAP) member 5; P glycoprotein ABCB5

Documentation

User Guide for Antibodies (1077 KB)