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PF4 Antibody (YA3156)

(Synonyms: C-X-C motif chemokine 4; CXCL4; Iroplact; OncostatinA; PF4; Platelet factor 4; SCYB4; short form; Small inducible cytokine subfamily member 4)
Customer Review

Based on 1 Customer Validation

PF4 Antibody (YA3156) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PF4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • PF4 Antibody (YA3156) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

PF4 Antibody (YA3156) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PF4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 11 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 11 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PF4 aa87-100.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105522

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PF4 Antibody (YA3156)
    Western blot analysis of extracts from A549 (lane2, 20μg), HL-60 (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Rat bone tissue (lane5, 20μg) using PF4 Antibody (HY-P83411). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PF4 Antibody (YA3156)
    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PF4 Antibody (YA3156)
    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PF4 Antibody (YA3156)
    Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PF4 Antibody (YA3156)
    Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PF4 is a Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence. Chemotactic for neutrophils and monocytes via CCR1. Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis. In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition. Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity[1][2][3][4][5][6][7].

  • Subcellular Localization

    Secreted

  • Expression


    Induction:By inflammatory conditions

  • Subunit

    Homotetramer (PubMed:7547867, PubMed:8031770). Interacts with TNFAIP6 (via Link domain) (PubMed:27044744). Interacts with CCR1 (PubMed:29930254). Interacts with CXCR3 (PubMed:18174362). Interacts with THBD; this interaction enhances generation of activated protein C (PubMed:9395524)

  • SwissProt ID

    P02776

  • Gene ID
    5196 [NCBI]
  • Synonyms

    C-X-C motif chemokine 4; CXCL4; Iroplact; OncostatinA; PF4; Platelet factor 4; SCYB4; short form; Small inducible cytokine subfamily member 4

  • Research Field

    Immunology

PF4 Antibody (YA3156) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2, 20μg), HL-60 (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Rat bone tissue (lane5, 20μg) using PF4 Antibody (HY-P83411). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PF4 Antibody (YA3156) Product Image
    1/16
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • PF4 Antibody (YA3156) Product Image
  • Experimental Validation Results for PF4 Antibody (YA3156)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2, 20μg), HL-60 (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Rat bone tissue (lane5, 20μg) using PF4 Antibody (HY-P83411). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PF4 Antibody (YA3156)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PF4 Antibody (YA3156)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PF4 Antibody (YA3156)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PF4 Antibody (YA3156)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • Experimental Validation Results for PF4 Antibody (YA3156)
  • PF4 Antibody (YA3156) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane2, 20μg), HL-60 (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Rat bone tissue (lane5, 20μg) using PF4 Antibody (HY-P83411). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • PF4 Antibody (YA3156) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PF4 Antibody (YA3156) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using PF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PF4 Antibody (YA3156) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PF4 Antibody (YA3156) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PF4 with PF4 Antibody (YA3156) (HY-P83411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PF4 Antibody (YA3156) (HY-P83411) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PF4 Antibody (YA3156) Product Image WB
  • PF4 Antibody (YA3156) Product Image IHC-P
  • PF4 Antibody (YA3156) Product Image IHC-P
  • PF4 Antibody (YA3156) Product Image ICC
  • PF4 Antibody (YA3156) Product Image ICC