PREB Antibody (YA1729)
(Synonyms: Prolactin regulatory element-binding protein; Mammalian guanine nucleotide exchange factor mSec12)
Based on 1 Customer Validation
PREB Antibody (YA1729) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PREB.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, ICC/IF, IP
-
Reactivity :
Human
-
Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
|
|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:50-1:100 | 1:100-1:200 | 1:20-1:50 |
Product Details
PREB Antibody (YA1729) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PREB.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 45 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 45 kDa
Recombinant protein of human PREB
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
PREB is a Guanine nucleotide exchange factor (GEF) that regulates the assembly of the coat protein complex II/COPII in endoplasmic reticulum (ER) to Golgi vesicle-mediated transport. Selectively activates SAR1A and SAR1B by promoting the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) in these small GTPases. In their activated GTP-bound state, SAR1A and SAR1B insert into the membrane of the endoplasmic reticulum where they recruit the remainder of the coat protein complex II/COPII which is responsible for both the sorting of proteins and the deformation and budding of membranes into vesicles destined to the Golgi; Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription[1].
-
Subcellular Localization
Endoplasmic reticulum membrane; Single-pass membrane protein; Nucleus
-
Expression
Tissue_specificity:Ubiquitous -
Subunit
Interacts with SAR1B (GDP-bound form) (By similarity). Interacts with MIA2; recruits PREB to endoplasmic reticulum exit sites (PubMed:25202031, PubMed:27170179). Interacts with CIDEB; facilitating loading of SCAP-SREBP into COPII vesicles (By similarity)
-
SwissProt ID
-
Synonyms
Prolactin regulatory element-binding protein; Mammalian guanine nucleotide exchange factor mSec12
-
Research Field
Epigenetics and Nuclear Signaling
Documentation
Powered by Bioz
