RAP1A+RAP1B Antibody (YA1824)(PBS only)
(Synonyms: Ras-related protein Rap-1b; K-REV; RAL1B)RAP1A+RAP1B Antibody (YA1824) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP1A+RAP1B.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20-1:50 | 1:20-1:50 |
Product Details
RAP1A+RAP1B Antibody (YA1824) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP1A+RAP1B.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 21 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 21 kDa
A synthetic peptide of human RAP1A/B
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using RAP1A+RAP1B Antibody (HY-P82079) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. -
Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), HUVEC (lane 3, 15 μg), HT-1080 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using RAP1A + RAP1B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Background
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Function
RAP1A+RAP1B is a GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function
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Subcellular Localization
Cell membrane; Cytoplasm, cytosol; Cell junction
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Isoforms & Post-Translational Modification
P61224 has 4 isomers: P61224-1: 20825 Da (predicted); P61224-2: 15356 Da (predicted); P61224-3: 18778 Da (predicted); P61224-4: 16033 Da (predicted).
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Subunit
Heterodimer with RAP1GAP (PubMed:18309292). Interacts with EPAC2 (PubMed:18660803). Interacts with SGSM1 (PubMed:17509819). Interacts with SGSM2 (PubMed:17509819). Interacts with SGSM3 (PubMed:17509819). Interacts with KRIT1 (PubMed:22577140). Interacts with RAP1GDS1 (PubMed:24415755)
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SwissProt ID
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Synonyms
Ras-related protein Rap-1b; K-REV; RAL1B
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Research Field
Signal Transduction
Documentation
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