WRN Antibody (YA2021)

Cat. No.: HY-P82276: Fiche technique COA
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User Guide for Antibodies
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WRN Antibody (YA2021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to WRN.

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Size	Stock	Quantité
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WRN Antibody (YA2021) Recommandations en vedette:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

WRN Antibody (YA2021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to WRN.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Masse moléculaire

Predicted band size: 162 kDa;

Observed band size: 200 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q14191

Gene ID

7486 [NCBI]

Immunogen

A synthesized peptide derived from human WRN aa1350-1432.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Pureté	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed:9224595, PubMed:9288107, PubMed:9611231). Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang (PubMed:11863428). Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA (PubMed:11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed:9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed:9224595, PubMed:9288107, PubMed:9611231). Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail (PubMed:10212265). d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound (PubMed:10212265). Depletion leads to chromosomal breaks and genome instability (PubMed:33199508)

Subcellular Localization：Nucleus, nucleolus; Nucleus; Nucleus, nucleoplasm; Chromosome

Subunit：Monomer, and homooligomer (PubMed:11863428, PubMed:18596042, PubMed:20159463). May exist as homodimer, homotrimer, homotetramer and/or homohexamer (PubMed:11863428, PubMed:18596042, PubMed:20159463). Homotetramer, or homohexamer, when bound to DNA (PubMed:11863428, PubMed:18596042, PubMed:20159463). Interacts via its N-terminal domain with WRNIP1 (By similarity). Interacts with EXO1, PCNA and SUPV3L1 (PubMed:12704184, PubMed:17961633). Interacts with PML (isoform PML-4) (PubMed:21639834). Interacts (via KBM motif) with XRCC5 and XRCC6; promoting recruitment to DNA damage sites (PubMed:27063109). Interacts with RECQL5; this interaction stimulates WRN helicase activity on DNA fork duplexes (PubMed:23180761)

RRID

AB_3104388

Database

Entrez Gene: 7486 Human

SwissProt: Q14191 Human

OMIM: 277700 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Exonuclease WRN; RecQ3; RECQL2; RECQL3; Werner syndrome helicase; WRN

Documentation

Select Batch:

Fiche technique (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

WRN Antibody (YA2021) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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WRN Antibody (YA2021)

WRN Antibody (YA2021) Recommandations en vedette:

Product Detail

Background

Description

WRN Antibody (YA2021) Related Classifications

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