Adenosine Deaminase Antibody (YA9756)
(Synonyms: ada, ADA1)Adenosine Deaminase Antibody (YA9756) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to Adenosine Deaminase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS (pH 7.4) , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 | 1:20-50 |
Product Details
Adenosine Deaminase Antibody (YA9756) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to Adenosine Deaminase.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Calculated Molecular Weight Predicted band size: 40.7 kDa;
Recombinant protein of human Adenosine Deaminase.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4) , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Adenosine Deaminase catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development (By similarity). Also responsible for the deamination of cordycepin (3'-deoxyadenosine), a fungal natural product that shows antitumor, antibacterial, antifungal, antivirus, and immune regulation properties[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Cell membrane; Cell junction; Cytoplasmic vesicle lumen; Cytoplasm; Lysosome
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Expression
Tissue_Specificity: Found in all tissues, occurs in large amounts in T-lymphocytes. Expressed at the time of weaning in gastrointestinal tissues.
Induction: Up-regulated by hypoxia. -
Isoforms & Post-Translational Modification
Adenosine Deaminase has an amino acid length of 363, molecular weight is 40764 Da.
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Subunit
Interacts with DPP4 (via extracellular domain).
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SwissProt ID
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Synonyms
ada, ADA1
Documentation
References
[1]. Eltzschig HK, et al. Endothelial catabolism of extracellular adenosine during hypoxia: the role of surface adenosine deaminase and CD26. Blood. 2006 Sep 1;108(5):1602-10. [Content Brief]
[2]. Gracia E, et al. The catalytic site structural gate of adenosine deaminase allosterically modulates ligand binding to adenosine receptors. FASEB J. 2013 Mar;27(3):1048-61. [Content Brief]
[3]. Fattahi A, et al. The Role of G22 A Adenosine Deaminase 1 Gene Polymorphism and the Activities of ADA Isoenzymes in Fertile and Infertile Men. Urology. 2015 Oct;86(4):730-4. [Content Brief]
[4]. Lindley ER, et al. Demonstration of adenosine deaminase activity in human fibroblast lysosomes. Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):457-62. [Content Brief]
[5]. Jiang C, et al. An adenosine deaminase (ADA) allele contains two newly identified deleterious mutations (Y97C and L106V) that interact to abolish enzyme activity. Hum Mol Genet. 1997 Dec;6(13):2271-8. [Content Brief]
[6]. Martinez-Navio JM, et al. Adenosine deaminase potentiates the generation of effector, memory, and regulatory CD4+ T cells. J Leukoc Biol. 2011 Jan;89(1):127-36. [Content Brief]
[7]. Ginés S, et al. Regulation of epithelial and lymphocyte cell adhesion by adenosine deaminase-CD26 interaction. Biochem J. 2002 Jan 15;361(Pt 2):203-9. [Content Brief]
[8]. Gonzalez-Gronow M, et al. Cell surface adenosine deaminase binds and stimulates plasminogen activation on 1-LN human prostate cancer cells. J Biol Chem. 2004 May 14;279(20):20993-8. [Content Brief]
[9]. Rostampour F, et al. Adenosine deaminase activity in fertile and infertile men. Andrologia. 2012 May;44 Suppl 1:586-9. [Content Brief]
[10]. Li G, et al. Inhibition of adenosine deaminase (ADA)-mediated metabolism of cordycepin by natural substances. Pharmacol Res Perspect. 2015 Mar;3(2):e00121. [Content Brief]
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