The Role of G22 A Adenosine Deaminase 1 Gene Polymorphism and the Activities of ADA Isoenzymes in Fertile and Infertile Men

  • Urology. 2015 Oct;86(4):730-4. doi: 10.1016/j.urology.2015.06.034.
Amir Fattahi  1 ,  Iraj Khodadadi  2 ,  Iraj Amiri  3 ,  Zeinab Latifi  4 ,  Marzieh Ghorbani  2 ,  Heidar Tavilani  5
Affiliations
  • 1. Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 2. Department of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
  • 3. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
  • 4. Department of Clinical Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 5. Urology & Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: [email protected].
PMID: 26166670 DOI: 10.1016/j.urology.2015.06.034
Abstract

Objective: To evaluate frequency distribution of Adenosine Deaminase 1 (ADA1) G22 A alleles and genotypes in fertile and infertile men.

Methods: In this study we evaluate frequency distribution of ADA1 G22 A alleles and genotypes in 200 fertile and 200 infertile men. The polymerase chain reaction-restriction fragment length polymorphism technique was used for determining ADA1 G22 A variants. In addition, ADA isoenzymes activities (ADA1 and ADA2) were measured using colorimetric method.

Results: The frequency of GG genotype was significantly higher and GA genotype was lower in infertile males compared with fertile men (P = .048 and P = .045, respectively). However, there was not any noticeable difference in allele distribution between groups (P >.05). Based on logistic regression analysis, the GA genotype has a protective role and can decrease the risk of male infertility 1.7 times (P = .046). There were significantly higher activities of ADAT and its isoenzymes in infertile males compared with fertile men (P <.05). Also, the ADA1 activity with GG genotype was higher than GA carriers in all population (P = .001).

Conclusion: Our results revealed that the activity of ADA isoenzymes and distribution of ADA1 G22 A genotypes were different among fertile and infertile men and more likely the GA genotype, which had lower ADA1 activity and was higher in fertile men is a protective factor against infertility.