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Cathepsin D Antibody (YA6245)

(Synonyms: CTSD; CPSD; Cathepsin D)
Customer Review

Based on 1 Customer Validation

Cathepsin D Antibody (YA6245) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cathepsin D.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Cathepsin D Antibody (YA6245) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC Info
 
WB Info
WB: Western Blot
IF Info
 
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Cathepsin D Antibody (YA6245) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cathepsin D.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human pancreas using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Cathepsin D is a lysosomal aspartic protease whose major described function is intracellular catabolism in lysosomal compartments, with additional roles in hormone processing and antigen processing[1]. Its function extends beyond nonspecific acidic bulk degradation, because cathepsin D-deficient mice maintain lysosomal bulk proteolysis but develop intestinal mucosal atrophy, lymphoid-cell destruction, and early death, indicating roles in limited proteolysis and tissue homeostasis[2]. Mechanistically, cathepsin D participates in endocytic and autophagic pathways where lysosomal proteases regulate substrate degradation and cellular remodeling[3]. In apoptosis-related models, mature lysosomal cathepsin D can translocate to the cytosol, and cathepsin D activity has been linked to induced apoptosis[4]. In breast cancer biology, cathepsin D is overexpressed and hypersecreted by epithelial breast cancer cells, correlates with clinical metastasis, and stimulates tumorigenicity, metastasis, cancer-cell proliferation, fibroblast outgrowth, and angiogenesis[4]. Compared with cathepsin E, cathepsin D showed distinct cytosolic activity after lysosomal permeabilization, because cathepsin D silencing prevented stefin B degradation whereas cathepsin E silencing did not[5]. For experimental applications, pepstatin A inhibited aspartic-protease-dependent stefin B degradation, and fluorogenic peptide probes detected cathepsin D activity in macrophages challenged with bacteria[5][6].

  • Subcellular Localization

    Lysosome; Melanosome; Secreted, extracellular space

  • Expression


    Tissue_specificity:Expression in the extracellular space of aortic cells (protein level) (PubMed: 20551380) . Expression in the liver (protein level) (PubMed: 1426530) .

  • Subunit

    Consists of a light chain and a heavy chain (PubMed:1426530, PubMed:8393577). Interacts with ADAM30; this leads to activation of CTSD (PubMed:27333034).

  • SwissProt ID

    P07339

  • Gene ID
    1509 [NCBI]
  • Synonyms

    CTSD; CPSD; Cathepsin D

References

[1]. Fusek M, et al. Dual role of cathepsin D: ligand and protease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Jun;149(1):43-50. [Content Brief]

[2]. Saftig P, et al. Mice deficient for the lysosomal proteinase cathepsin D exhibit progressive atrophy of the intestinal mucosa and profound destruction of lymphoid cells. EMBO J. 1995 Aug 1;14(15):3599-608. [Content Brief]

[3]. Müller S, et al. Specific functions of lysosomal proteases in endocytic and autophagic pathways. Biochim Biophys Acta. 2012 Jan;1824(1):34-43. [Content Brief]

[4]. Liaudet-Coopman E, et al. Cathepsin D: newly discovered functions of a long-standing aspartic protease in cancer and apoptosis. Cancer Lett. 2006 Jun 18;237(2):167-79. [Content Brief]

[5]. Železnik TZ, et al. Aspartic cathepsin D degrades the cytosolic cysteine cathepsin inhibitor stefin B in the cells. Biochem Biophys Res Commun. 2015 Sep 18;465(2):213-7. [Content Brief]

[6]. Rodriguez-Rios M, et al. A fluorogenic, peptide-based probe for the detection of Cathepsin D in macrophages. Commun Chem. 2023 Nov 2;6(1):237. [Content Brief]

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Cathepsin D Antibody (YA6245) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    1/16
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Cathepsin D Antibody (YA6245) Product Image
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Experimental Validation Results for Cathepsin D Antibody (YA6245)
  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Cathepsin D Antibody (HY-P86553, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin D Antibody (YA6245) Product Image IHC-P
  • Cathepsin D Antibody (YA6245) Product Image IHC-P
  • Cathepsin D Antibody (YA6245) Product Image IHC-P
  • Cathepsin D Antibody (YA6245) Product Image IHC-P
  • Cathepsin D Antibody (YA6245) Product Image IHC-P
  • Cathepsin D Antibody (YA6245) Product Image IHC-P
  • Cathepsin D Antibody (YA6245) Product Image IHC-P
  • Cathepsin D Antibody (YA6245) Product Image IHC-P