Adipose Triglyceride Lipase Antibody (YA6009)
Based on 1 Customer Validation
Adipose Triglyceride Lipase Antibody (YA6009) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adipose Triglyceride Lipase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
Adipose Triglyceride Lipase Antibody (YA6009) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adipose Triglyceride Lipase.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 55 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Adipose Triglyceride Lipase catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Also possesses acylglycerol transacylase and phospholipase A2 activities. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Regulates adiposome size and may be involved in the degradation of adiposomes. Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes. Acts antagonistically with LDAH in regulation of cellular lipid stores. Inhibits LDAH-stimulated lipid droplet fusion. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Lipid droplet; Cell membrane; Multi-pass membrane protein; Cytoplasm
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Expression
Tissue_specificity:It is expressed most strongly in adipose tissue. It has also been detected in the heart, skeletal muscle, and parts of the gastrointestinal tract. It can be detected in both normal retina and retinoblastoma cells. It is also detected in the retinal pigment epithelium, with lower expression levels (protein levels) in photoreceptor ganglia and the neuroretinal ganglion cell layer. -
Isoforms & Post-Translational Modification
Q96AD5 has 2 isomers: Q96AD5-1: 55316 Da (predicted); Q96AD5-2: 19875 Da (predicted).
Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity (By similarity). Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation (PubMed:22733971);Ubiquitinated by PEX2 in response to reactive oxygen species (ROS), leading to its degradation (PubMed:34903883). Ubiquitination is stimulated by LDAH (PubMed:28578400) -
Subunit
Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity (By similarity). Interacts with SERPINF1; this interaction stimulates the phospholipase A2 activity of PNPLA2 (PubMed:17032652). Despite a colocalization in lipid droplets, it probably does not interact with PLIN (By similarity). Interacts with PLIN5; prevents interaction with ABHD5 (By similarity). Interacts with FAF2 (PubMed:23297223)
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SwissProt ID
Documentation
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