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ZFP91 Antibody (YA4429)

(Synonyms: PZF; DMS-8; DSM-8; FKSG11; ZFP-91; ZNF757)
Customer Review

Based on 1 Customer Validation

ZFP91 Antibody (YA4429) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ZFP91.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • ZFP91 Antibody (YA4429) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

ZFP91 Antibody (YA4429) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ZFP91.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70-100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Immunogen

Purified recombinant fragment of human ZFP91 (AA: 162-304) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718973

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), A431 (lane 3(20μg), HepG2 (lane 4(20μg) and HEK293 (lane 5(20μg) using ZFP91 Antibody (HY-P84732) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    Western blot analysis of extracts from COS-7(lane 1(40μg) )
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    Flow cytometric analysis of 1X10^6 Hela cells labeling ZFP91 Antibody(HY-P84732
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    Immunocytochemistry analysis of A549 cells labeling ZFP91 Antibody (HY-P84732) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZFP91 Antibody (HY-P84732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    Immunofluorescence analysis of Hela cells labeling ZFP91 antibody (HY-P84732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    ZFP91 is an Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific

  • Isoforms & Post-Translational Modification

    Q96JP5 has 2 isomers: Q96JP5-1: 63445 Da (predicted); Q96JP5-2: 59571 Da (predicted).

  • Subunit

    Interacts with MAP3K14/NIK

  • SwissProt ID

    Q96JP5

  • Gene ID
    80829 [NCBI]
  • Synonyms

    PZF; DMS-8; DSM-8; FKSG11; ZFP-91; ZNF757

ZFP91 Antibody (YA4429) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), A431 (lane 3(20μg), HepG2 (lane 4(20μg) and HEK293 (lane 5(20μg) using ZFP91 Antibody (HY-P84732) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from COS-7(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ZFP91 Antibody(HY-P84732

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ZFP91 Antibody (HY-P84732) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZFP91 Antibody (HY-P84732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling ZFP91 antibody (HY-P84732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • ZFP91 Antibody (YA4429) Product Image
    1/16
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • ZFP91 Antibody (YA4429) Product Image
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), A431 (lane 3(20μg), HepG2 (lane 4(20μg) and HEK293 (lane 5(20μg) using ZFP91 Antibody (HY-P84732) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from COS-7(lane 1(40μg) )
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ZFP91 Antibody(HY-P84732
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ZFP91 Antibody (HY-P84732) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZFP91 Antibody (HY-P84732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling ZFP91 antibody (HY-P84732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • Experimental Validation Results for ZFP91 Antibody (YA4429)
  • ZFP91 Antibody (YA4429) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), A431 (lane 3(20μg), HepG2 (lane 4(20μg) and HEK293 (lane 5(20μg) using ZFP91 Antibody (HY-P84732) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ZFP91 Antibody (YA4429) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from COS-7(lane 1(40μg) )

  • ZFP91 Antibody (YA4429) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling ZFP91 Antibody(HY-P84732

  • ZFP91 Antibody (YA4429) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling ZFP91 Antibody (HY-P84732) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZFP91 Antibody (HY-P84732) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ZFP91 Antibody (YA4429) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling ZFP91 antibody (HY-P84732) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • ZFP91 Antibody (YA4429) Product Image WB
  • ZFP91 Antibody (YA4429) Product Image WB
  • ZFP91 Antibody (YA4429) Product Image FC
  • ZFP91 Antibody (YA4429) Product Image ICC
  • ZFP91 Antibody (YA4429) Product Image ICC