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RBP4 Antibody (YA100)

(Synonyms: PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP)
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Based on 1 Customer Validation

RBP4 Antibody (YA100) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RBP4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RBP4 Antibody (YA100)
  • RBP4 Antibody (YA100) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:50-200 1:100-500 1:50-100

Product Details

Description

RBP4 Antibody (YA100) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RBP4.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 23kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23kDa
Species Reactivity Database

Entrez Gene: 5950 Human

SwissProt: P02753 Human

Immunogen

A synthesized peptide derived from human Retinol binding protein 4. The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RBP4 Antibody (YA100)
    Western blot analysis was performed on protein extracts from HepG2 (lane 2, 20 μg) and HepG2 (lane 3, 40 μg) using RBP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    RBP4 is a Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane[1][2].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It can be detected in both plasma and urine (measured at the protein level) .

  • Subunit

    Interacts with TTR (PubMed:10052934, PubMed:19021760, PubMed:5541771, PubMed:7754382). Interaction with TTR prevents its loss by filtration through the kidney glomeruli (Probable). Interacts with STRA6 (PubMed:22665496, PubMed:25910211)

  • SwissProt ID

    P02753

  • Gene ID
    5950 [NCBI]
  • Synonyms

    PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP

References

[1]. Peterson PA, et al. Studies on the interaction between prealbumin, retinol-binding protein, and vitamin A. J Biol Chem. 1971 Jan 10;246(1):44-9. [Content Brief]

[2]. Berry DC, et al. Cross talk between signaling and vitamin A transport by the retinol-binding protein receptor STRA6. Mol Cell Biol. 2012 Aug;32(15):3164-75. [Content Brief]

RBP4 Antibody (YA100) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from HepG2 (lane 2, 20 μg) and HepG2 (lane 3, 40 μg) using RBP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • RBP4 Antibody (YA100) Product Image
    1/16
  • Experimental Validation Results for RBP4 Antibody (YA100)
  • RBP4 Antibody (YA100) Product Image
  • Experimental Validation Results for RBP4 Antibody (YA100)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from HepG2 (lane 2, 20 μg) and HepG2 (lane 3, 40 μg) using RBP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for RBP4 Antibody (YA100)
  • RBP4 Antibody (YA100) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts from HepG2 (lane 2, 20 μg) and HepG2 (lane 3, 40 μg) using RBP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • RBP4 Antibody (YA100) Product Image WB