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Phospho-MLKL (Ser345) Antibody (YA7438)

(Synonyms: Mixed lineage kinase domain-like protein, hMLKL, MLKL)
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Phospho-MLKL (Ser345) Antibody (YA7438) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-MLKL (Ser345).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Phospho-MLKL (Ser345) Antibody (YA7438) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

Phospho-MLKL (Ser345) Antibody (YA7438) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-MLKL (Ser345).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    MLKL is a Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm,Cell membrane,Nucleus

  • Isoforms & Post-Translational Modification

    Q8NB16 has two isomers: Q8NB16-1: 54479 Da (predicted); Q8NB16-2: 30279 Da (predicted).
    Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis (PubMed:22265413)

  • Subunit

    Homooligomer (PubMed:29883610)

  • SwissProt ID

    Q8NB16

  • Synonyms

    Mixed lineage kinase domain-like protein, hMLKL, MLKL

Phospho-MLKL (Ser345) Antibody (YA7438) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Phospho-MLKL (Ser345) Antibody (YA7438) Product Image
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  • Phospho-MLKL (Ser345) Antibody (YA7438) Product Image