CD43 Antibody (YA802)

Cat. No.: HY-P80061: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CD43 Antibody (YA802) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD43.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD43 Antibody (YA802) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CD43 Antibody (YA802) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD43.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 41 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P16150

Gene ID

6693 [NCBI]

Immunogen

Synthetic peptide within C-terminal human CD43.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Protein G affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using CD43 Antibody (HY-P80061) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity); Protects cells from apoptotic signals, promoting cell survival

Subcellular Localization：Membrane; Single-pass type I membrane protein; Cell projection, microvillus; Cell projection, uropodium; Nucleus; Nucleus, PML body

Expression：
Tissue_specificity:thymocytes, T lymphocytes, neutrophils, plasma cells, and the surface of myeloma cells

Subunit：Interacts with SIGLEC1

RRID

AB_3102812

Database

Entrez Gene: 6693 Human

SwissProt: P16150 Human

OMIM: 182160 Human

Research Field

Immunology

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CD43 Antibody (YA802) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD43 Antibody (YA802)

CD43 Antibody (YA802) Featured Recommendations:

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CD43 Antibody (YA802) Related Classifications

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