AUP1 Antibody (YA4599)(PBS only)

Cat. No.: HY-P84902A: Data Sheet User Guide for Antibodies
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AUP1 Antibody (YA4599) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to AUP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

AUP1 Antibody (YA4599) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to AUP1.

Host

Mouse

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9Y679

Gene ID

550 [NCBI]

Immunogen

Purified recombinant fragment of human AUP1 (AA: 229-410) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome (PubMed:18711132, PubMed:21857022). Plays a role in lipid droplet formation (PubMed:21857022). Induces lipid droplet clustering (PubMed:24039768). Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation (PubMed:21127063, PubMed:23223569). Also required for the degradation of INSIG1, SREBF1 and SREBF2 (PubMed:23223569). Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB (PubMed:28183703); (Microbial infection) Following Dengue virus infection, required for induction of lipophagy which facilitates production of virus progeny particles

Subcellular Localization：Endoplasmic reticulum membrane; Peripheral membrane protein; Lipid droplet; Cytoplasmic vesicle, autophagosome

Expression：
Tissue_specificity:Detected in blood platelets and leukocytes (at protein level) . Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain

Induction: (Microbial infection) By Dengue virus infection (at protein level)

Isoforms & Post-Translational Modification：Q9Y679 has 2 isomers: Q9Y679-2: 45787 Da (predicted); Q9Y679-3: 41385 Da (predicted).
Monoubiquitinated and diubiquitinated;(Microbial infection) Not ubiquitinated following Dengue virus infection

Subunit：Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1 (PubMed:18711132). Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM (PubMed:12042322). Interacts (via C-terminus) with ubiquitin-conjugating enzyme UBE2G2; the interaction recruits UBE2G2 to lipid droplets (PubMed:21127063, PubMed:23223569). Interacts with ubiquitin ligases AMFR/gp78 and RNF139/TRC8; this promotes interaction of UBE2G2 with AMFR and RNF139 (PubMed:23223569). Interacts with apolipoprotein APOB (PubMed:28183703)

Synonyms

Lipid droplet-regulating VLDL assembly factor AUP1, Ancient ubiquitous protein 1, AUP1

Documentation

User Guide for Antibodies (1077 KB)