AUP1 Antibody (YA4599)(PBS only)
(Synonyms: Lipid droplet-regulating VLDL assembly factor AUP1, Ancient ubiquitous protein 1, AUP1)AUP1 Antibody (YA4599) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to AUP1.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:10000 |
Product Details
AUP1 Antibody (YA4599) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to AUP1.
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Host Mouse
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 46 kDa
Purified recombinant fragment of human AUP1 (AA: 229-410) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG2b
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
AUP1 plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2. Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB; (Microbial infection) Following Dengue virus infection, required for induction of lipophagy which facilitates production of virus progeny particles[1][2][3][4][5][6].
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Subcellular Localization
Endoplasmic reticulum membrane; Peripheral membrane protein; Lipid droplet; Cytoplasmic vesicle, autophagosome
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Expression
Tissue_specificity:Detected in blood platelets and leukocytes (at protein level) . Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain
Induction: (Microbial infection) By Dengue virus infection (at protein level) -
Isoforms & Post-Translational Modification
Q9Y679 has 2 isomers: Q9Y679-2: 45787 Da (predicted); Q9Y679-3: 41385 Da (predicted).
Monoubiquitinated and diubiquitinated;(Microbial infection) Not ubiquitinated following Dengue virus infection -
Subunit
Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1 (PubMed:18711132). Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM (PubMed:12042322). Interacts (via C-terminus) with ubiquitin-conjugating enzyme UBE2G2; the interaction recruits UBE2G2 to lipid droplets (PubMed:21127063, PubMed:23223569). Interacts with ubiquitin ligases AMFR/gp78 and RNF139/TRC8; this promotes interaction of UBE2G2 with AMFR and RNF139 (PubMed:23223569). Interacts with apolipoprotein APOB (PubMed:28183703)
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SwissProt ID
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Synonyms
Lipid droplet-regulating VLDL assembly factor AUP1, Ancient ubiquitous protein 1, AUP1
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